广西柳州市丙型肝炎病毒的基因型和基因亲缘性分析  

Viral genotypes and phylogenesis of hepatitis C virus in Liu Zhou, Guangxi

作　　者：尹琦[1,2] 杨庆利[1] 王学燕[1] 李海[1] 陈钦艳[1] 谭超[1] 罗洲[2] 

机构地区：[1]广西壮族自治区疾病预防控制中心,广西病毒性肝炎防制研究重点实验室,广西南宁530028 [2]柳州市柳铁中心医院,广西柳州545007

出　　处：《中国热带医学》2016年第10期965-969,共5页China Tropical Medicine

摘　　要：目的分析近年来广西柳州市丙型肝炎病毒(HCV)的基因型和基因亲缘性。方法用巢式RT-PCR法对HCV NS5B区进行扩增,通过测序和构建进化树进行基因分型并分析基因亲缘性。结果对182株HCV基因分型:1a型占3.3%,1b型占50.5%,3a型占17.0%,3b型占4.9%,6a型占26.4%,6c/d型占2.7%,6型未分亚型(6un)占0.5%。6株1a型HCV与美国参照株属不同分支。2.2%(2/92)的1b型与日本参照株属同一分支;29.3%(27/92)的1b型与来源于上海的参照株属于同一分支,其余67.4%(62/92)的1b型则独立分支;另外1株独立于上述各分支外。95.2%(20/21)的3a型以及77.8%(7/9)的3b型与日本和澳大利亚参照株属同一分支或并列分支。4.8%(1/21)的3a型和22.2%(2/9)的3b型则独立分支。97.9%(47/48)的6a型与越南、香港和英国参照株属同一分支或并列分支;2.1%(1/48)的6a型独立于上述分支外。5株HCV与6c和6d参照株并列分支,1株6型病毒未能区分亚型。结论近年来广西柳州市HCV流行的主导基因型是1b、6a和3a。1b型的大部分具有独立的本地亲缘性特征;而6a和3a型的绝大部分则无此特征。Objective To analyze the genotypes and genetic affinity of the strains of hepatitis C virus（HCV） isolated inLiuzhou, Guangxi in recent late years. Methods The NS5 B region of the viruses was amplified based by nested RT-PCR.And the genetic affinity was analyzed by sequencing and establishing the phylogenetic tree. The phylogenesis of the viruseswere also analyzed. Results The genotypes of 182 strains of HCV consisted of 3.3% of 1a, 50.5% of 1b, 17.0% of 3a, 4.9% of3 b, 26.4% of 6a, 2.7% of 6c/d, and 0.5 % of unclassified（6un）, respectively. The 6 strains of genotype 1a belonged to differentbranches with the U.S. reference strains. And 2.2%（2/92） of genotype 1b strains were at the same branch with the Japanreference strains, while 29.3%（27/92） of 1b at the same branch with the Shanghai reference strain, and 67.4%（62/92） of 1bbranched independently. Only 1 strain of genotype 1b was located outside of the branches mentioned above. For genotype 3HCV, 95.2%（20/21） of genotype 3a strains and 77.8%（7/9） of genotype 3b strains belonged to the same or parallel brancheswith the Japan and Australia reference strains, while 4.8%（1/21） of genotype 3a and 22.2%（2/9） of genotype 3b strainsbranched independently. A predominant 97.9%（47/48） of genotype 6a strains belonged to the same or parallel branches withthe Vietnam, Hongkong, and U.K. reference strains, while 2.1%（1/48） of genotype 6a was outside of the branches. There were 5strains of HCV paralleled with the branches of 6c and 6d reference strains. And 1 strain of genotype 6 HCV failed to subtyping.Conclusion The major genotypes of HCV prevalent in Liuzhou in recent years are 1b, 6a, and 3a. Most of the genotype 1bstrains show the independent local genetic phylogeny. However, the majority of genotype 3a and 6a strains don＇t have suchfeatures.

关 键 词：丙型肝炎病毒 基因型 亲缘性分析 

分 类 号：R373.2[医药卫生—病原生物学]