高效液相色谱法测定青稞红曲中内酯式和酸式洛伐他汀的含量  

Content determination of lovastatin and lovastatin acid in highland barley Monascus by HPLC

作　　者：陈发贵 张丹 刘敏 熊燕 张森林 赵辉 

机构地区：[1]青藏高原微生物国家地方联合工程中心,西藏拉萨850000 [2]西藏月王生物技术有限公司,西藏拉萨850000

出　　处：《中国酿造》2016年第10期162-165,共4页China Brewing

摘　　要：采用高效液相色谱法测定青稞红曲中内酯和酸式洛伐他汀的含量,以体积分数75%的乙醇作为提取剂,在室温条件下超声提取青稞红曲中的洛伐他汀1 h。使用Eclipse Plus C_(18)柱(5μm,250 mm×4.6 mm),调整柱温30℃,流动相为甲醇∶0.1%磷酸=73∶27;流速1.0 mL/min;进样量20μL;紫外检测器波长238 nm。内酯式洛伐他汀标准品溶液在20~100μg/m L时呈良好的线性(r^2=0.999 7),内酯式洛伐他汀的平均回收率为99.30%,相对标准偏差为1.67%;酸式洛伐他汀标准品溶液在20~100μg/m L时呈良好的线性(r^2=0.999 9),酸式洛伐他汀的平均回收率为98.50%,相对标准偏差为1.65%。因此高效液相色谱法能够简便、快捷、准确测定青稞红曲中内酯式和酸式洛伐他汀的含量。Lovastatin and lovastatin acid in the highland barley Monascus were determined by HPLC. The sample was extracted using 75% ethanol as extraction solvent at room temperature for 1 h, then detected by HPLC method with Eclipse Plus C18 column （5 μm, 250 mm×4.6 mm） at column temperature 30 ℃, methanol-0.1% phosphate solution （73：27） as mobile phase, flow rate 1.0 ml/min, sample quantity 20 μl and detection wavelength 238 nm. The linear range of lovastatin was 20-100 μg/ml （r2=0.9997）, the mean recovery was 99.30%, and the relative standard deviation was 1.67%. While the linear range oflovastatin acid was 20-100 μg/ml （r2=-0.999 9）, the mean recovery was 98.50%, and the relative standard deviation was 1.65%. The HPLC method to determine the content of lovastatin and lovastatin acid in the highland barley Monascus was convenient, rapid and accurate.

关 键 词：青稞红曲 洛伐他汀 酸式洛伐他汀 高效液相色谱 

分 类 号：O657.7[理学—分析化学]