Effect of dietary interventions on the intestinal microbiota of Mongolian hosts  

作　　者：Jing Li Haiyan Xu Zhihong Sun Qiangchuan Hou Lai-Yu Kwok Wuri Laga Yanjie Wang Huimin Ma Zhongjie Yu Bilige Menghe Heping Zhang 

机构地区：[1]Key Laboratory of Dairy Biotechnology and Engineering,Ministry of Education,Inner Mongolia Agricultural University

出　　处：《Science Bulletin》2016年第20期1605-1614,共10页科学通报（英文版）

摘　　要：The gut microbiota of Mongolian hosts has distinctive characteristics due to their meat- and dairyoriented daily diets and unique genotype.The aim of the present study was to investigate the effect of switching from the typical high protein and fat Mongolian diets to carbohydrate-rich meals composed principally of wheat,rice and naked oats on the host gut microbiota within 3 weeks.Our study took the advantage of the long sequence reads produced by the Pac Bio single molecule real-time sequencing technology to enable the profiling of subjects' gut microbiota communities along the diet intervention to the species precision.We found that the bacterial richness and diversity decreased apparently along the diet intervention.During the diet intervention,the gut microbiota composition displayed no significant difference at phylum level(with major phyla of Firmicutes,Bacteroidetes,Tenericutes and Proteobacteria).The relative abundances of some genera such as Bacteroidetes,Faecalibacterium,Roseburia,Alistipes,Streptococcus,and Oscillospira were significantly altered after the diet switching started.Notably,significant changes were also observed in the proportions of the species Bacteroides dorei,Bacteroides fragilis,Bacteroides thetaiotaomicron,Ruminococcus albus,Ruminococcus faecis,Roseburia faecis and Eubacterium ventriosum.These results have demonstrated that diet and host gut microbiota is closely linked.The gut microbiota of Mongolian hosts has distinctive characteristics due to their meat- and dairy- oriented daily diets and unique genotype. The aim of the present study was to investigate the effect of switching from the typical high protein and fat Mongolian diets to carbohydrate-rich meals composed principally of wheat, rice and naked oats on the host gut microbiota within 3 weeks. Our study took the advantage of the long sequence reads produced by the PacBio single molecule real-time sequencing technology to enable the profiling of subjects＇ gut microbiota communities along the diet intervention to the species precision. We found that the bacterial richness and diversity decreased apparently along the diet intervention. During the diet intervention, the gut microbiota composition displayed no significant difference at phylum level （with major phyla of Firmicutes, Bac- teroidetes, Tenericutes and Proteobacteria）. The relative abundances of some genera such as Bacteroidetes, Fae- calibacterium, Roseburia, Alistipes, Streptococcus, and Oscillospira were significantly altered after the diet switching started. Notably, significant changes were also observed in the proportions of the species Bacteroides dorei, Bacteroides fragilis, Bacteroides thetaiotaomicron, Ruminococcus albus, Ruminococcus faecis, Roseburiafaecis and Eubacterium ventriosum. These results have demonstrated that diet and host gut microbiota is closely linked.

关 键 词：Gut microbiota Diet intervention MONGOLIAN Pac Bio single molecule real-time sequencing technology(SMRT sequencing) 

分 类 号：TS201.3[轻工技术与工程—食品科学]