Sensitive detection of dopamine and quinone drugs based on the quenching of the fluorescence of carbon dots  

作　　者：Xiangling Ren Jiejie Ge Xianwei Meng Xiaozhong Qiu Jun Ren Fangqiong Tang 

机构地区：[1]Laboratory of Controllable Preparation and Application of Nanomaterials, Key Laboratory of Photochemical Conversion and Optoelectronic Materials, Technical Institute of Physics and Chemistry, Chinese Academy of Sciences, Beijing 100190,China [2]Department of Anatomy, Guangdong Provincial Key Laboratory of Construction and Detection in Tissue Engineering, Southern Medical University, Guangzhou 510515, China

出　　处：《Science Bulletin》2016年第20期1615-1623,共9页科学通报（英文版）

基　　金：supported by the National Natural Science Foundation of China (61178035,61571426,61671435,81671845,81630053,51428301,and 31572343);the National High Technology R&D Program of China (2015BAI23H01);Beijing Natural Science Foundation (4161003)

摘　　要：In this work,we demonstrated that the quinone structure can quench the fluorescence of the carbon dots(CDs).The sensitive determination of dopamine(DA) was studied primarily based on this principle.DA would be transformed into DA quinone under alkaline conditions,which resulted in fluorescence quenching of the CDs.A good linear range from 5 nmol/L to 0.4 mmol/L was obtained and the detection limit was 1 nmol/L.Moreover,the quenching effect of quinone structure on the fluorescence of CDs was confirmed by Fourier transform infrared spectra,time-correlated single-photon counting and X-ray photoelectron spectroscopy.Remarkably,CDs were firstly applied to detect the quinone drugs quantitatively which contained typical quinone structure based on the quenching mechanism.More than this,the sensing platform was demonstrated to provide credible selectivity and satisfactory stability in human serum solution with good liner range.Hence,our practical application and mechanism have showed great potential for diagnostic purposes.In this work, we demonstrated that the quinone structure can quench the fluorescence of the carbon dots （CDs）. The sensitive determination of dopamine （DA） was studied primarily based on this principle. DA would be transformed into DA quinone under alkaline conditions, which resulted in fluorescence quenching of the CDs. A good linear range from 5 nmol/L to 0.4 mmol/L was obtained and the detection limit was 1 nmol/L. Moreover, the quenching effect of quinone structure on the fluores- cence of CDs was confirmed by Fourier transform infrared spectra, time-correlated single-photon counting and X-ray photoelectron spectroscopy. Remarkably, CDs were firstly applied to detect the quinone drugs quantitatively which contained typical quinone structure based on the quenching mechanism. More than this, the sensing platform was demonstrated to provide credible selectivity and satisfac- tory stability in human serum solution with good linerrange. Hence, our practical application and mechanism have showed great potential for diagnostic purposes.

关 键 词：Carbon dots Quinone structure Detection DOPAMINE Quinone drugs 

分 类 号：R927[医药卫生—药学] O657.3[理学—分析化学]