丹参蒽醌的提取工艺及抑菌活性研究  

Extraction and antimicrobial activity of anthraquinone in Salvia miltiorrhiza Bge.

作　　者：任雪峰[1,2] 齐亚娥[1,2] 宋海[1,2] 谢复腾 吴冬青[1,2] 安红钢[1,2] REN Xue-feng QI Ya-e SONG Hai XIE Fu-teng WU Dong-qing AN Hong-gang(College of Chemistry and Chemical Engineering, Hexi University, Zhangye Gansu 734000 Key Laboratory of Hexi Corridor Resources Utilization of Gansu, Zhangye Gansu 734000, China)

机构地区：[1]河西学院化学化工学院,甘肃张掖734000 [2]甘肃省河西走廊特色资源利用重点实验室,甘肃张掖734000

出　　处：《中兽医医药杂志》2016年第5期30-34,共5页Journal of Traditional Chinese Veterinary Medicine

基　　金：甘肃省河西走廊特色资源利用重点实验室(XZ-1008)资助项目

摘　　要：在单因素试验的基础上,采用响应面分析法优化丹参中蒽醌的提取工艺,并采用琼脂稀释法测定丹参中蒽醌的抑菌活性。结果表明,丹参中蒽醌提取最佳工艺条件为:提取时间2 h,提取温度70℃,料液比1∶9(g/m L)。在此条件下,丹参中蒽醌的提取率可达0.466%。抑菌试验表明蒽醌对金黄色葡萄球菌、沙门氏菌和大肠杠菌的最小抑菌浓度分别为0.625 mg/m L、0.156 mg/m L和1.25 mg/m L。Based on single-factor experiments, response surface analysis methodology（RSM） was used to optimize the extraction technology of anthraquinone from Salvia miltiorrhiza Bge.. Along with this, the inhibition of anthraquinone aganist common bacteria was investigated. The results showed the optimal extraction conditions were extraction temperature of 70 ℃, material-to-liquid ratio of 1 ∶9 g·m L^（-1） and extraction time was 2 h. Under these conditions, extraction rate of anthraquinone was up to 0.466%.Antimicrobial activity test showed the minimal inhibitory concentration（MIC） against Staphylococcus aureus, Salmonella and Escherichia coli was 0.625, 0.156 and 1.25 mg·m L^（-1）, respectively.

关 键 词：丹参 蒽醌 提取工艺 抑菌性 

分 类 号：S853.7[农业科学—临床兽医学]