细菌性软腐菌对人参根系分泌物中糖类和氨基酸类的化学趋向性响应  

Chemotaxis response of Erwinia carotovora on sugars and amino acids of root exudates of Panax ginseng

作　　者：张爱华[1] 安宁波[1] 雷锋杰[2] 马文丽[1] 匙坤 张连学[1] 

机构地区：[1]吉林农业大学中药材学院吉林省人参工程技术研究中心,吉林长春130118 [2]沈阳农业大学植物保护学院,辽宁沈阳110866 [3]延边州工业和信息化局,吉林延边133000

出　　处：《中国中药杂志》2016年第21期3937-3941,共5页China Journal of Chinese Materia Medica

基　　金：国家自然科学基金项目(31100239;31200224;31470420);吉林省科技发展计划项目(20140520159JH)

摘　　要：采用毛细管法测定了人参细菌性软腐菌在4种趋化参数(浓度、时间、温度和pH)下对不同氨基酸和糖的趋向性响应。与对照组结果显示,赖氨酸趋化率为2.509 7(pH 8,质量浓度为0.025 mg·L^(-1)、培养温度为25℃、培养时间为60 min),精氨酸趋化率为2.218 8(pH 6、质量浓度为0.25 mg·L^(-1)、培养温度为25℃、培养时间为60 min),L-鼠李糖趋化率为3.091 2(pH 7、质量浓度为0.25 mg·L^(-1)、培养温度为30℃、培养时间为60 min)和D-阿拉伯糖趋化率为3.026 3(pH 6、质量浓度为0.25 mg·L^(-1)、培养温度为30℃、培养时间为45 min)。糖类和氨基酸类对人参细菌性软腐菌有明显的趋化作用,且高浓度的氨基酸和糖对人参细菌性软腐菌的化学趋向性有抑制作用,但趋化作用随浓度的减弱反而增加。The chemotaxis response of Erwinia carotovora to different sugars and amino acids in four kinds of chemotactic parameters（ concentration,time,temperature and pH） was determined by capillary method. The results showed that when pH was 8,concentration was 0. 025 mg·L^（-1）,culture temperature was 25 ℃ and the duration was 60 minutes,the optimal chemotaxis rate of lysine was 2. 509,when pH was 6,concentration was 0. 25 mg·L^（-1）,culture temperature was 25 ℃ and the duration was 60 minutes,the optimal chemotaxis rate of arginine was 2. 218 8,when pH was 7,concentration was 0. 25 mg·L^（-1）,culture temperature was 30 ℃ and the duration was 60 minutes,the optimal chemotaxis rate of L-rhamnose was 3. 091 2,when pH was 6,concentration was 0. 25 mg·L^（-1）,culture temperature was 30 ℃ and the duration was 45 minutes,the optimal chemotaxis rate of D-arabinose was 3. 026 3. Sugars and amino acids had obvious chemotaxis with E. carotovora,the high concentration of carbohydrate and amino acid exited an inhibitory effect on chemotaxis response of E. carotovora,and the chemotaxis response decreased with the increase of concentration of carbohydrates and amino acids.

关 键 词：人参 氨基酸 糖 细菌性软腐菌 化学趋向性 

分 类 号：S435.675[农业科学—农业昆虫与害虫防治]