蒙药嘎日迪-13对脑缺血大鼠不同时相海马中IL-1β IL-8、ICAM-1、P-selectin表达的影响  

Influence of mongolian medicine garidi-13 on the expression of IL-1β、IL-8、ICAM-1、P-selectin at different phase of cerebral ischemic rats hippocampus

作　　者：张浩楠[1] 郑延泽[1] 何静波[1] 田彩云[1] 武海军[1] 杨玉梅[1] 

机构地区：[1]包头医学院药学院,内蒙古包头014000

出　　处：《中风与神经疾病杂志》2016年第10期905-908,共4页Journal of Apoplexy and Nervous Diseases

基　　金：国家自然科学基金项目(No.81160560)

摘　　要：目的观察蒙药嘎日迪-13对脑缺血大鼠不同时相海马中IL-1β、IL-8、ICAM-1、P-selectin表达的影响。方法 360只雄性SD大鼠随机分为脑缺血模型组、假手术组、嘎日迪-13大、中、小剂量组,每组又随机分为6个时相组,灌胃给药,一次/d,连续27 d后,线栓法制备大鼠大脑中动脉栓塞模型,神经功能评分后免疫组化法测定海马IL-1β、IL-8、ICAM-1、P-selectin的表达。结果嘎日迪-13明显降低脑缺血大鼠神经功能评分,并明显降低脑缺血大鼠海马IL-1β、IL-8、ICAM-1、P-selectin的表达水平。结论蒙药嘎日迪-13对大鼠脑缺血有较好的保护作用,其机制可能与降低IL-1β、IL-8、ICAM-1、P-selectin等因子的表达水平有关。Objective To observe the influence of Mongolian Medicine Garidi-13 on the expression of IL-1β、IL-8、ICAM-1、P-selectin at different phase of cerebral ischemic rats hippocampus .Methods 360 male SD rats were randomly divided into vehicle control group ,sham-operated group ,Gridi-13 large dose group ,Gridi-13 middle dose group and Gridi-13 small dose group,then each group was divided into the ischemia 1 h,6 h,12 h,24 h,72 h and 120 h phases.Lavage rats with Garidi-13 once a day till 27th,then established cerebral ischemia model with Suture method cerebral ischemia model . After having recorded the neurofunctional score ,their brain samples were collected for immunohistochemical staining of IL-1β, IL-8,ICAM-1 and P-selectin.Result The expressions of hippocampal IL-1β,IL-8,ICAM-1 and P-selectin in the model group were significantly higher than those in vehicle group （P〈0.01）.According to the results of immunohistochemical staining,the Garidi-13 groups could inhibit the expression of IL-1β,IL-8,ICAM-1,P-selectin.Conclusion Garidi-13’ s protective mechanism for brain tissues of cerebral ischemic rats may be related to its effects in inhibiting IL -1β,IL-8,ICAM-1,P-selectin expression.

关 键 词：蒙药 嘎日迪-13 脑缺血 大鼠 IL-1Β IL-8 ICAM-1 P-SELECTIN 

分 类 号：R743.3[医药卫生—神经病学与精神病学]