食管上皮高级别内瘤变与食管鳞癌组织中第2个线粒体衍生的半胱氨酸蛋白酶激活剂和生存素的表达及意义  

Expressions and significance of the second mitochondria-derived activator of caspase protein and Survivin in tissues of high-grade intraepithelial neoplasia and esophageal squamous cell carcinoma

作　　者：马臻奇[1] 陈凤娟[1] 沈丽娟[1] 王学红[1] 王丰梅[2] 马雪芹[1] 马旭翔[1] 绽永华[1] 王芳[1] 郭新建[2] 

机构地区：[1]青海大学附属医院消化内科,西宁810001 [2]青海大学附属医院病理科,西宁810001

出　　处：《中华消化外科杂志》2016年第11期1106-1112,共7页Chinese Journal of Digestive Surgery

基　　金：青海大学附属医院中青年基金（ASRF-2011-05）

摘　　要：目的：通过检测第2个线粒体衍生的半胱氨酸蛋白酶激活剂（Smac）和生存素（Survivin）在食管上皮高级别内瘤变和食管鳞癌患者组织中的表达，探讨Smac和Survivin对食管鳞癌发生、发展的影响。方法：采用回顾性病例对照研究方法。收集2012年1月至2014年12月青海大学附属医院收治的 45例健康体检者（可疑食管炎者）设为对照组，41例高级别食管内瘤变患者设为高级别食管内瘤变组， 57例食管鳞癌患者设为食管鳞癌组的临床病理资料。观察指标：（1）免疫组织化学染色检测Smac和 Survivin的表达。（2）RTPCR检测食管鳞癌组织和癌旁组织中Smac和Survivin mRNA的表达。（3）分析食管鳞癌组织和癌旁组织中Smac和Survivin相对表达量的相关性。（4）食管鳞癌患者食管鳞癌组织中Smac和Survivin表达与临床病理因素的关系。正态分布的计量资料采用±s表示，多组间比较采用单因素方差分析；癌组织和癌旁组织比较采用配对t检验。率的比较采用χ^2检验或Fisher确切概率法。癌组织中Smac和Survivin表达量行Pearson相关性分析。结果：（1）免疫组织化学染色检测Smac和Survivin的表达：对照组、高级别食管内瘤变组、食管鳞癌组受试者食管组织中Smac阳性表达率分别为82.2%（37/45）、43.9%（18/41）、40.4%（23/57），3组比较，差异有统计学意义（χ^2=20.408，P〈0.05）。对照组分别与高级别食管内瘤变组、食管鳞癌组比较，差异均有统计学意义（χ^2=13.665，18.202，P〈0.05）；高级别食管内瘤变组与食管鳞癌组比较，差异无统计学意义（χ^2=0.124，P〉0.05）。食管鳞癌组患者癌旁组织中Smac阳性表达率为84.2%（48/57），与食管鳞癌组织阳性表达率比较，差异有统计学意义（χ^2=20.530，P〈0.05）。对照组、高级别食管内瘤变组、食管鳞癌组受试者食管组织中Survivin阳性表达率分别为11.1%（5/45�Objective：To detect the expressions of the second mitochondriaderived activator of caspase protein（Smac） and Survivin in tissues of highgrade intraepithelial neoplasia（HGIN）and esophageal squamous cell carcinoma （ESCC）, and explore the influence of Smac and Survivin on occurrence and development of ESCC. Methods：The retrospective casecontrol study was conducted. The clinicopathological data of 45 healthy controls with suspected esophagitis （control group）, 41 patients with HGIN （HGIN group） and 57 patients with ESCC （ESCC group） who were admitted to the Qinghai University Affiliated Hospital between January 2012 and December 2014 were collected. Observation indicators included： （1） expressions of Smac and Survivin were detected by immunohistochemistry. （2） Expressions of mRNA of Smac and Survivin in tumor tissues and adjacent tissues were detected by realtime polymerase chain reaction （RTPCR）. （3） Correlation between relative expressions of Smac and Survivin in tumor tissues and adjacent tissues. （4） Relationship between expressions of Smac and Survivin in tumor tissues and clinicopathological factors. Measurement data with normal distribution were represented as ±s. The comparisons among groups was evaluated with the oneway ANOVA. The comparison between tumor tissues and adjacent tissues were analyzed by the paired t test, and comparison of rates was done using the chisquare test and Fisher exact probability. The correlation between expressions of Smac and Survivin was done using the Pearson correlation analysis. Results：（1） Expressions of Smac and Survivin were detected by immunohistochemistry. The positive expression rate of Smac of esophageal tissues in the control group, HGIN group and ESCC group were respectively 82.2%（37/45）, 43.9%（18/41） and 40.4%（23/57）, with a statistically significant difference among the 3 groups （χ^2=20.408, P〈0.05）. There were statistically significant differences in the positive expression rate of Smac be

关 键 词：食管肿瘤 线粒体促凋亡蛋白 生存素蛋白 食管上皮高级别内瘤变 

分 类 号：R735.1[医药卫生—肿瘤]