前列腺上皮内瘤基底细胞变化的形态学观察  

Morphology of Prostatic Intraepithelial Neoplasia Basal Cell Changes

作　　者：邢兆辉[1] 王齐[1] 

机构地区：[1]黑龙江省医院泌尿外科,黑龙江哈尔滨150036

出　　处：《黑龙江医学》2016年第10期916-918,共3页Heilongjiang Medical Journal

摘　　要：目的研究观察前列腺上皮内瘤基底细胞的形态学变化特点,为前列腺病变以及鉴别诊断提供可靠依据。方法观察前列腺上皮内瘤基底细胞,采用特异性标记高分子角蛋白抗体(34βE12)、鼠抗人P63蛋白前列腺特异性抗原(PSA)、前列腺特异性抗体(PSAP)、甲酰基辅酶A消旋酶(P540S)检测,应用光镜和免疫组织化染色Elivision二步法。结果肿瘤病灶周围基底细胞完全缺失4例,占16%,基底细胞续断存在12例,占48%,9例基底细胞完整,占36%,其中基底细胞完全缺失P504S为阴性,基底细胞续断存在中有3例为P504S阳性。结论前列腺上皮内瘤基底细胞可完整存在,可续断存在,也可缺失,基底细胞的完整与否不能作为PIN与前列腺癌的诊断标准,应综合应用细胞形态学指标,降低漏诊率和误诊率。Objective Morphological changes characteristic of basal cell tumor of the prostate epithelium were observed in the study,as well as to provide a reliable basis for the differential diagnosis of lesions before the forefront. Methods Patients with tumors in the hospital were retrospectively analyzed,and prostatic intraepithelial neoplasia basal cells were observed using specific markers polymer keratin antibodies（ 34βE12）,mouse anti-human P63 protein and prostate-specific antigen（ PSA）,prostate-specific antibody（ PSAP）,A acyl-coenzyme A racemase（ P540S） detected by light microscopy and immunohistochemical staining Elivision two footwork. Results PIN cell immunohistochemical expression show： 4 cases basal cell cancer lesions around the complete absence,accounting for 16%; 12 cases have basal cell Dipsacus,accounting for 48%; 9 cases have basal cell integrity,accounting for 36%,including negative basal cell completely missing P504 S,basal cell Dipsacus existing in three cases for P504 S positive. Conclusion Prostatic intraepithelial neoplasia can complete,exist teaselly and miss the presence of basal cell. Basal cell or not can not be used as a complete diagnostic criteria PIN and prostate cancer,should be integrated application of morphological indicators,and reduce the rate of misdiagnosis and misdiagnosis rate.

关 键 词：前列腺上皮内瘤 基底细胞 前列腺癌 形态学 

分 类 号：R737.25[医药卫生—肿瘤]