电针改变CFA炎症痛大鼠前扣带回脑区神经元放电活动  

ELECTRO-ACUPUNCTURE MODULATES THE NEURONAL FIRINGS OF ANTERIOR CINGULATE CORTEX IN RATS WITH INFLAMMATORY PAIN

作　　者：周萌萌[1] 刘风雨[1] 岳路鹏 蔡捷[1] 廖斐斐[1] 朱兵[2] 景向红[2] 万有[1,3,4] 伊鸣[1] ZHOU Meng-Meng LIU Feng-Yu YUE Lu-Peng CAI Jie LIAO Fei-Fei ZHU Bing JING Xiang-Hong WAN You YI Ming(Neuroscience Research Institute Department of Neurobiology, School of Basic Medical Sciences Key Lab for Neuroscience, Ministry of Education/National Health and Family Planning Commission, Peking University, Beijing 100191 Institute of Acupuncture and Moxibustion, China Academy of Chinese Medical Sciences, Beijing 100700, China)

机构地区：[1]北京大学神经科学研究所,北京100191 [2]中国中医科学院针灸研究所,北京100700 [3]北京大学基础医学院神经生物学系,北京100191 [4]北京大学教育部/国家卫生计生委神经科学重点实验室,北京100191

出　　处：《中国疼痛医学杂志》2016年第10期737-742,共6页Chinese Journal of Pain Medicine

基　　金：科技部“973”项目(2013CB531905,2014CB548200,2015CB554503);国家自然科学基金项目(31200835);中国中医科学院针灸研究所开放选题项目(ZZ04004)

摘　　要：目的:研究电针对炎症痛大鼠前扣带回(anterior cingulate cortex,ACC)神经元放电的影响。方法:实验大鼠分为4组:CFA炎症痛模型组加电针,CFA炎症痛模型组加假电针,对照组加电针,对照组加假电针。应用多通道在体记录技术,记录在电针前、后1 h内以及给予激光痛刺激前、后ACC神经元的放电,处理记录到的神经信号并进行统计分析。结果:电针后,CFA炎症痛组和对照组大鼠ACC神经元的平均放电率均增高,CFA炎症痛组大鼠ACC脑区内对激光痛刺激有反应的兴奋性神经元反应性降低。结论:电针激活炎症痛大鼠ACC脑区的神经元,但抑制ACC脑区内对痛刺激起兴奋性反应的神经元。推测电针通过调节ACC脑区神经元活动而镇痛。Objective： To investigate the effect of electro-acupuncture on neuronal firings in the anterior cingulate cortex（ACC） in inflammatory pain rats induced by intraplantar injection of complete Freund＇s adjuvant（CFA）. Methods： Male SD rats were divided into four groups： CFA group with electro-acupuncture, CFA group with sham electro-acupuncture, Control group with electro-acupuncture, and Control group with sham electro-acupuncture. The neuronal firings in the ACC were recorded before and after electro-acupuncture, with noxious heat stimulation by CO2 laser stimulator. The neuronal firings were processed with Mat Lab and Neuro Explorer, and analyzed with softwares Graph Pad Prism 5.0 and SPSS. Results： Electro-acupuncture increased the mean firing rate of ACC neurons in both CFA and Control groups, but decreased the standardized value of peak firing rate of neurons activated by noxious heat. Conclusion： Electro-acupuncture activated ACC neurons, and inhibited the excitatory responses of ACC neurons to noxious heat.

关 键 词：炎症痛 前扣带回 电针 神经元放电 

分 类 号：R245[医药卫生—针灸推拿学]