丘脑腹内侧核高频电刺激改善帕金森病模型大鼠前肢使用不对称行为的研究  

High-frequency stimulation of the ventromedial thalamic nucleus improves limb-use asymmetry in hemiparkinsonian rats

作　　者：潘琪[1] 张旺明[2] 肖宗宇[3] 罗非[4] 徐如祥 Pan Qi Zhang Wangming Xiao Zongyu et al(Affiliated hospital of Hainan Medical University, Haikou, 570102)

机构地区：[1]海南医学院附属医院神经外科,海口5701021 [2]南方医科大学珠江医院神经外科 [3]青海大学附属医院神经外科 [4]中国科学院心理研究所 [5]陆军总医院附属八一脑科医院

出　　处：《立体定向和功能性神经外科杂志》2016年第3期129-133,共5页Chinese Journal of Stereotactic and Functional Neurosurgery

基　　金：国家自然科学基金面上项目(编号:30872662);海南医学院科研培育基金项目(编号:HY2015-19)

摘　　要：目的探讨丘脑腹内侧核高频电刺激能否改善帕金森病模型大鼠前肢使用不对称行为。方法将33只SD大鼠随机分为实验组22只和对照组11只。利用立体定向的方法将刺激电极埋入右侧丘脑腹内侧核,用于注射6-羟基多巴胺的套管埋入右侧内侧前脑束。实验组大鼠右侧内侧前脑束内注射12μg的6-OHDA,对照组注射相同体积的生理盐水。对造模前、造模后和给予DBS时的大鼠进行前肢使用不对称测试。黑质酪氨酸羟化酶免疫组化染色检测黑质多巴胺能神经元毁损程度。尼氏染色确定刺激电极尖端位置。结果从22只大鼠中获得了15只成功的PD大鼠模型。与毁损前相比,模型大鼠毁损后的不对称指数显著增大(P=0.000)。给予高频VM-DBS后,不对称指数显著下降,与毁损后未给予VM-DBS时比较,差异有统计学意义(P=0.009);而与毁损前相比,差异不显著(P=0.214)。给予低频VM-DBS后,不对称指数无明显改变,与毁损后未给予VM-DBS时比较,差异无统计学意义(P=0.4);而与毁损前相比,差异显著(P=0.036)。结论丘脑腹内侧核高频电刺激能够改善6-羟基多巴胺偏侧帕金森病模型大鼠的前肢使用不对称行为,丘脑腹内侧核可能是帕金森病深部脑刺激治疗的潜在有效靶核团。Objective To investigate whether high-frequency stimulation of the ventromedial thalamic nucleus can improve limb-use asymmetry in rats with unilateral 6-hydroxydopamine lesions.Methods Male Sprague-Dawley rats were assigned to 2groups：6-OHDA group（n=22）and control group（n=11）.A stimulation electrode was implanted into the right ventromedial thalamic nucleus（VM）.A microinjection cannula was also implanted ipsilateral to the electrode.The unilateral 6-OHDA rat model was induced by injection of 6-OHDA into unilateral medial forebrain bundle.Limb-use asymmetry assessment was investigated.Low（25Hz）or high（130Hz）stimulation of the VM（pulse width 60μs,50~175μA）was delivered during behavioral tests.Results Fifteen out of the 22 rats were successfully lesioned after the injection of 6-OHDA into MFB.In model rats,compared with the score before lesion,the limb-use asymmetry score increased significantly（P=0.000）after lesion.Compared with the score without DBSVM,the limb-use asymmetry score during HFS-VM decreased significantly（P=0.009）.However,the score during LFS-VM show no difference（P=0.4）.Conclusion High-frequency stimulation of the ventromedial thalamic nucleus improves limb-use asymmetry in rats with unilateral 6-hydroxydopamine lesions.The ventromedial thalamic nucleus might be an effective tar-get for PD treatment.

关 键 词：丘脑腹内侧核 深部脑刺激 前肢使用不对称测试 6-羟基多巴胺 大鼠 

分 类 号：R745.2[医药卫生—神经病学与精神病学]