白血病患儿骨髓间充质干细胞与白血病细胞株K562/AO2生长增殖及凋亡的相关性  

Correlation between growth, proliferation and apoptosis of leukemia cell lines K562/AO2 and bone marrow mesenchymal stem cells in children with leukemia

作　　者：雷春霞[1] 

机构地区：[1]武汉市儿童医院新生儿内科,湖北省武汉市430016

出　　处：《中国组织工程研究》2016年第41期6112-6117,共6页Chinese Journal of Tissue Engineering Research

摘　　要：背景:骨髓微环境是白血病的发病根源,微环境的改变对白血病细胞的生物学行为和性能有一定的影响。目的:探讨白血病患儿骨髓间充质干细胞对K562/AO2细胞生长增殖及凋亡的影响。方法:体外环境下进行K562/AO2细胞单独悬浮培养,K562/AO2细胞与白血病患儿骨髓间充质干细胞共培养。锥虫蓝染色计数活细胞数,描绘两组细胞生长曲线,PI单染法检测细胞周期,Annexin V/PI法检测细胞凋亡情况。结果与结论:(1)单独培养组前3 d细胞生长未出现明显改变,第4天开始细胞增殖速度明显加快,并于培养第6天达到最高峰。共培养组整体增殖曲线较为平缓,无明显的增殖高峰;(2)单独培养组的G0-G1期细胞比例显著低于共培养组,S期细胞比例显著高于共培养组(P<0.05)。两组G2-M期细胞比例差异无显著性意义;(3)单独培养组的细胞凋亡数显著高于共培养组(P<0.05);(4)结果表明,与白血病患儿骨髓间充质干细胞共培养后K562/AO2细胞生长受到抑制,细胞周期阻滞在G0-G1期,且会对K562/AO2细胞凋亡产生抵抗作用。BACKGROUND： Leukemia comes from the damage to bone marrow microenvironment, and changes in bone marrow microenvironment lead to some effects on biological characteristics and behavior of leukemia cells. OBJECTIVE： To explore the effect of bone marrow mesenchymal stem cells from children with leukemia on K562/AO2 cell line growth, proliferation and apoptosis. METHODS： K562/AO2 cells were cultured alone in vitro, or co-cultured with bone marrow mesenchymal stem cells from leukemia children. The living cells in the two groups were counted using trypan blue staining, and the cell growth curves were described. PI single staining method was used to detect the cell cycle, and cell apoptosis was detected by V/PI Annexin method in the two groups. RESULTS AND CONCLUSION： After single suspension culture, the cells showed no marked changes within the first 3 days, but began to proliferate fast at 4 days and reached the proliferation peak at 6 days. Co-cultured cells showed a gentle growth curve with no presence of obvious proliferation peak. Compared with the co-culture group, the proportion of G0/G1 phase cells was significantly lower, but the proportion of S phase cells was significantly higher in the single culture group（P〈0.05）. The number of cells in the G2/M phase showed no significant difference between the two groups（P〉0.05）. All these findings indicate that co-culture with bone marrow mesenchymal stem cells from leukemia children inhibits the proliferation of K562/AO2 cells through G0/G1 phase arrest, and also resists apoptosis in K562/AO2 cells.

关 键 词：干细胞 骨髓干细胞 白血病 骨髓间充质干细胞 白血病细胞株 细胞增殖 细胞凋亡 细胞周期 

分 类 号：R394.2[医药卫生—医学遗传学]