来源于乳腺癌正常组织乳腺干细胞的安全性  

Safety assessment of mammary gland stem cells from normal tissues in breast cancer patients

作　　者：毕晓娟[1] 付明刚[2] 郭丽英[2] 刘莎[2] 迪丽米娜.伊拉木 郭晨明 

机构地区：[1]新疆医科大学第一附属医院临床医学研究院科研基地科,新疆维吾尔自治区乌鲁木齐市830054 [2]新疆医科大学第一附属医院消化血管中心乳腺外科,新疆维吾尔自治区乌鲁木齐市830054

出　　处：《中国组织工程研究》2016年第41期6125-6130,共6页Chinese Journal of Tissue Engineering Research

基　　金：新疆重大疾病医学重点实验室开放课题(SKLIB-XJMDR-ZX-2014-1);新疆医科大学第一附属医院自然科学基金青年项目(2013ZRQN50)~~

摘　　要：背景:乳腺干细胞的培养对研究乳腺的发育及乳腺癌具有重要的意义。目的:找到一种易操作的体外分离培养乳腺干细胞的方法,并验证其安全性。方法:分离培养乳腺癌旁正常组织中乳腺上皮细胞,流式细胞仪分选CD49f和EPCAM阳性细胞进行干细胞表面标记分析及细胞集落形成能力分析,然后在乳腺干细胞、乳腺癌组织和乳腺癌旁正常组织中提取mR NA,实时荧光定量PCR检测C-erbB-2和Maspin基因的表达。结果与结论:(1)成功培养出人乳腺干细胞,并且细胞表面特异性标志物CD49f,EPCAM高表达;(2)集落形成能力分析可见混合集落、腔上皮细胞集落、肌上皮细胞集落;(3)乳腺干细胞C-erbB-2低表达,而Maspin高表达,证明来源于乳腺癌正常组织的乳腺干细胞具有生物安全性。BACKGROUND： The cultivation of mammary gland stem cells is of great significance for the development of mammary gland and breast cancer. OBJECTIVE： To seek an easy method to isolate and culture mammary gland stem cell in vitro, and verify the safety of cells. METHODS： Mammary epithelial cells were isolated from normal tissues surrounding breast cancer, and CD49f- and EPCAM-positive cells were sorted using flow cytometry followed by surface marker analysis and cell colony formation ability analysis. Afterwards, real-time fluorescent quantitative PCR was used to detect C-erb B-2 and Maspin mR NA expression in mammary gland stem cells, breast cancer tissues and normal tissues surrounding breast cancer. RESULTS AND CONCLUSION： Human mammary gland stem cells were successfully cultured and highly expressed CD49 f and EPCAM, with the presence of mixed colony, pleural epithelial cell colony, and myoepithelial cell colony. c-erb B-2 was lowly expressed while Maspin highly expressed in mammary gland stem cells. Our experimental findings indicate that the mammary gland stem cells derived from normal tissue surrounding breast cancer have biological safety.

关 键 词：干细胞 肿瘤干细胞 乳腺干细胞 乳腺癌 C-ERBB-2 MASPIN CD49f EPCAM 

分 类 号：R394.2[医药卫生—医学遗传学]