三七皂苷联合骨髓间充质干细胞移植促进急性脑损伤的恢复  

Treatment of traumatic brain injury by Panax notoginseng saponins combined with bone marrow mesenchymal stem cell transplantation

作　　者：李雷兵[1] 王皎月[1] 孙彩虹[1] 

机构地区：[1]日照市人民医院重症医学科,山东省日照市276800

出　　处：《中国组织工程研究》2016年第41期6138-6144,共7页Chinese Journal of Tissue Engineering Research

摘　　要：背景:研究证明,三七皂苷治疗脑缺血损伤有着广泛的药理基础,骨髓间充质干细胞移植治疗脑损伤动物模型的实验研究也已广泛开展,但二者联用鲜有报道。目的:观察三七皂苷联合骨髓间充质干细胞移植促进大鼠急性脑损伤的恢复作用。方法:采用液压冲击法建立SD大鼠脑损伤模型,将60只SD大鼠随机分为脑损伤组、骨髓间充质干细胞移植组及骨髓间充质干细胞+三七皂苷联合治疗组。治疗后采用Bederson神经缺损评分评估神经功能恢复情况,Western blot法检测脑组织中神经生长因子蛋白表达变化,苏木精-伊红染色观察脑组织形态学变化,TUNEL法检测皮质神经元凋亡情况。结果与结论:(1)Bederson评分:脑损伤组>骨髓间充质干细胞移植组>联合治疗组,各组间比较差异有显著性意义(P<0.05);(2)神经生长因子蛋白表达:联合治疗组>骨髓间充质干细胞移植组>脑损伤组,各组间比较差异有显著性意义(P<0.05);(3)与脑损伤组及骨髓间充质干细胞移植组比较,联合治疗组脑组织炎症浸润细胞明显减少,水肿程度明显减轻;(4)与脑损伤组及骨髓间充质干细胞移植组比较,联合治疗组神经元凋亡细胞显著减少;(5)结果表明,三七皂苷联合骨髓间充质干细胞移植能够促进大鼠急性脑损伤后的神经功能恢复,其可能与促进神经生长因子表达及减少神经元细胞凋亡有关。BACKGROUND： Studies have shown that Panax notoginseng saponins（PNS） has extensive pharmacological basis for the treatment of cerebral ischemic injury, and animal experiments for treatment of brain injury by bone marrow mesenchymal stem cell transplantation are ongoing. However, little is reported about the combined use of PNS and bone marrow mesenchymal stem cell transplantation. OBJECTIVE： To investigate the effect of PNS combined with bone marrow mesenchymal stem cell transplantation on traumatic brain injury in rats. METHODS： Traumatic brain injury models were made in 60 Sprague-Dawley rats by hydraulic shock method. Then, model rats were randomized into model group, cell transplantation group receiving bone marrow mesenchymal stem cell transplantation and combined treatment group undergoing PNS combined with bone marrow mesenchymal stem cell transplantation. Nerve function recovery of the rats and protein expression of nerve growth factor in the brain tissue were evaluated and detected by Bederson scoring and western blot methods, respectively. Morphological changes of the brain tissue and apoptosis in cortical neurons were observed and detected by hematoxylin-eosin staining and TUNEL, respectively. RESULTS AND CONCLUSION： Highest Bederson score was found in the model group, followed by the cell transplantation group and combined treatment group（P〈0.05）, while the protein expression of nerve growth factor was ranked as follows： the combined treatment group the cell transplantation group the model group（P〈0.05）. Inflammatory infiltration and brain edema in the brain were relieved markedly in the combined treatment group compared with the other two groups, and the number of apoptotic neurons was significantly reduced as well. Our findings suggest that PNS combined with bone marrow mesenchymal stem cell transplantation can promote neurological recovery from traumatic brain injury by increasing the expression of nerve growth factor and reducing neuronal apoptosis.

关 键 词：干细胞 移植 三七皂苷 骨髓间充质干细胞移植 神经生长因子 大鼠 

分 类 号：R394.2[医药卫生—医学遗传学]