骨髓间充质干细胞移植对胃癌模型大鼠肿瘤细胞的影响  

Effect of transplantation of bone marrow mesenchymal stem cells on gastric cancer cell line SGC7901 in a rat model of gastric cancer

作　　者：陈超[1] 李海涛[2] 

机构地区：[1]遵化市人民医院病理科,河北省遵化市064200 [2]遵化市人民医院外一科,河北省遵化市064200

出　　处：《中国组织工程研究》2016年第41期6158-6163,共6页Chinese Journal of Tissue Engineering Research

摘　　要：背景：近年来研究发现,骨髓间充质干细胞对肿瘤组织具有靶向性迁移的特性。目的：探讨骨髓间充质干细胞移植对大鼠胃癌细胞趋向性及增殖分化的影响。方法：SD大鼠皮下注射胃癌细胞株SGC-7901建立胃癌模型,建模成功后细胞移植组腹腔注射荧光标记培养的骨髓间充质干细胞（1×10~7个/只）,移植后10 d采用荧光Di I标记检测骨髓间充质干细胞的靶向性,荧光实时PCR和Western blot检测胃癌组织中细胞周期素D2 mRNA和蛋白的表达,原位末端标记法染色观察胃癌细胞凋亡情况。结果与结论：（1）骨髓间充质干细胞成功靶向到大鼠胃癌组织;（2）细胞移植组的细胞周期素D2 mRNA及其蛋白的表达水平均显著高于模型组（P〈0.05）;（3）与模型组比较,细胞移植组阳性凋亡细胞明显减少（P〈0.05）;（4）结果表明,骨髓间充质干细胞具有靶向迁移到胃癌组织的能力,骨髓间充质干细胞移植可促进胃癌肿瘤细胞的增殖。BACKGROUND： Bone marrow mesenchymal stem cells can migrate into tumor tissues, as reported inrecent studies. OBJECTIVE： To investigate the tropism and effect of transplantation of bone marrow mesenchymal stem cells on proliferation and differentiation of gastric cancer cell line SGC7901 in rats. METHODS： Gastric cancer models were established in Sprague-Dawley rats by subcutaneous injection of gastric cancer cell line SGC7901, and then, in cell transplantation group, each rat underwent an intraperitoneal injection of 1×10~7 bone marrow mesenchymal stem cells. After transplantation, the targeting ability of bone marrow mesenchymal stem cells was detected using fluorescent Di I labeling. Cyclin D2 m RNA and protein expressions were measured using real-time PCR and western blot assay, respectively. Apoptosis of gastric cancer cells was observed by in situ terminal labeling method. RESULTS AND METHODS： Bone marrow mesenchymal stem cells successfully migrated into the gastric cancer site in rats. The expression levels of cyclin D2 mRNA and protein in the cell transplantation group were significantly higher than those in the model group（P〈0.05）. It was observed that the number of apoptotic cancer cells was significantly reduced in the cell transplantation group compared with the model group（P〈0.05）. These findings indicate that bone marrow mesenchymal stem cells have the ability to migrate into the tumor sites, thereby promoting the proliferation of gastric cancer cells.

关 键 词：干细胞 移植 胃癌 骨髓间充质干细胞 SGC-7901细胞 细胞凋亡 CYCLIND2 

分 类 号：R394.2[医药卫生—医学遗传学]