灌胃给予六味地黄丸后椎间盘退变组织蛋白多糖成分的变化  

Changes in proteoglycan components in degenerative intervertebral disc after intragastric administration of Liuwei Dihuang Wan

作　　者：王乐[1] 徐无忌[1] 

机构地区：[1]湖南中医药大学第二附属医院脊柱科,湖南省长沙市410005

出　　处：《中国组织工程研究》2016年第42期6271-6277,共7页Chinese Journal of Tissue Engineering Research

基　　金：国家自然科学基金青年科学基金项目(81302993);湖南中医药科技计划重点项目(201317)~~

摘　　要：背景:蛋白多糖、硫酸软骨素、硫酸角质素、透明质酸均是维持椎间盘正常结构的重要物质,影响整个椎间盘的生理功能。目的:观察六味地黄丸对兔椎间盘退变模型椎间盘蛋白多糖成分的影响,探讨六味地黄丸防治椎间盘退变的疗效。方法:将80只新西兰兔随机分为六味地黄丸组、模型组、假手术组、空白对照组,每组20只。模型组、六味地黄丸组予建立椎间盘退变动物模型;假手术组行相同手术入路进入腰椎,再依次缝合组织;六味地黄丸组给予六味地黄胶囊混悬液10 mg/kg灌胃,1次/d;模型组、假手术组、空白对照组灌予等量生理盐水,1次/d,自然喂养。于2,4,6,8周后每个时间点处死动物,每次每组处死5只。留取椎间盘标本,测量椎间盘细胞蛋白多糖成分。结果与结论:随着给药时间的延长,六味地黄丸能上调椎间盘退变模型中糖胺多糖含量、硫酸软骨素/硫酸角质素比值及透明质酸含量,从而稳定蛋白多糖含量,在一定程度上延缓椎间盘的退变。BACKGROUND：Proteoglycan, chondroitin sulfate and keratan sulfate, and hyaluronic acid are important substances in the maintenance of intervertebral discs of normal structure. They impact the physiological function of intervertebral disc. OBJECTIVE：To observe the influence of Liuwei Dihuang Wan on proteoglycan in rabbit models of intervertebral disc degeneration, and to study the efficacy of Liuwei Dihuang Wan on prevention and treatment of intervertebral disc degeneration. METHODS：80 New Zealand rabbits were randomly divided into Liuwei Dihuang Wan group, model group, sham surgery group and blank control group （n=20 per group）. Animal models of intervertebral disc degeneration were established in the model and Liuwei Dihuang Wan groups. In the sham surgery group, surgery via the same approach to expose the lumbar spine was conducted fol owed by layer-by-layer suturing. In the Liuwei Dihuang Wan group, rabbits were given 10 mg/kg Liuwei Dihuang Wan by lavage, once a day. In the model group, sham surgery group and blank control group, rabbits were given an equal volume of physiological saline, once a day, by natural feeding. At 2, 4, 6 and 8 weeks, five rabbits from each group were sacrificed, and intervertebral disc specimens were taken to measure proteoglycan components in the intervertebral disc. RESULTS AND CONCLUSION：With the extension of feeding time, Liuwei Dihuang Wan could increase sugar amino polysaccharide content, chondroitin sulfate/acid ratio of keratin, and hyaluronic acid content in the intervertebral disc degeneration model, and stabilized the proteoglycan content. Thus, it delayed the degeneration of the intervertebral disc to a certain extent.

关 键 词：动物 模型 椎间盘退化 软骨素硫酸盐类 透明质酸 组织工程 组织构建 软骨组织工程 椎间盘退变 蛋白多糖 糖胺多糖 硫酸软骨素 硫酸角质素 国家自然科学基金 

分 类 号：R318[医药卫生—生物医学工程]