孤养对成年大鼠认知行为与不同脑区突触相关蛋白表达的影响  

Effects of social isolation on cognitive behavior and the expression of synapse-associated proteins in different brain regions of adult rats

作　　者：任庆国[1] 公卫刚 

机构地区：[1]东南大学附属中大医院神经内科,南京210009

出　　处：《中华行为医学与脑科学杂志》2016年第10期877-881,共5页Chinese Journal of Behavioral Medicine and Brain Science

基　　金：国家自然科学基金项目（91232707）;江苏省普通高校研究生创新计划资助项目（KYZZ16_0132）

摘　　要：目的探讨孤养对成年大鼠认知行为、不同脑区结构及突触相关蛋白表达的影响。方法24只成年SD大鼠随机分为群养组（4只／笼）和孤养组（1只／笼），每组12只。6周后使用Morris水迷宫检测大鼠空间学习与记忆能力；运用基于体素的形态学分析（voxel-based morphometry，VBM）评估脑结构改变；应用蛋白免疫印迹（Western blot）检测不同脑区突触相关蛋白（synaptophysin）和PSD93的表达。结果水迷宫结果显示孤养组训练第五天潜伏期较群养组明显延长[（21．49±1．38）s vs（16．64±1．70）s，P〈0．05]，同时孤养组穿梭平台次数和目标象限百分比较群养组均明显降低[（1．91±0．25）次vs（3．08±0．31）次，P〈0．01；（28．39±1．70）％vs（36．14±2．89）％，P〈0．05]，差异有统计学意义。与群养大鼠相比，孤养大鼠脑内海马、杏仁核、听觉皮层、压后皮层等脑区的体素显著降低；孤养大鼠海马突触相关蛋白synaptophysin和PSD93表达较群养组明显降低[synaptophysin：（0．61±0．03）vs（1．18±0．07），P〈0．05；PSD93：（0．54±0．03）体（1．36±0．08），P〈0．01]，孤养大鼠杏仁核突触相关蛋白PSD93表达较群养组明显减少[（0．97±0．08）vs（1．63±0．2），P〈0．01]。结论孤养会诱导成年大鼠视空间学习记忆能力损害，海马和杏仁核脑区萎缩，突触相关蛋白synaptophysin和PSD93表达减少可能是孤养诱导其认知损害的病理基础。Objective To investigate the effect of the social isolation on the cognitive behavior and the structure as well as the expression of synapse-associated proteins in different brain regions in adult rats. Methods Twenty-four Sprague-Dawley adult rats （10 months） were divided into group-housed group （ 4 per cage） and isolation group （ 1 per cage）.Six-weeks later, Morris water maze test, voxel-based morphometry （VBM） and Western-blot were used to detect cognitive function, brain structure and pathological changes of synapse respectively. Results In the Morris water maze test, compared with the group-housed animals, the escaping latency in social isolation group prolonged （ （ 21.49±1.38） s vs （ 16.64±1.70） s, P〈0.05 ） on day 5 was significantly. Meanwhile, compared with the group-housed animals, the percentage of time spent in target quadrant and the number of target crossings in social isolation group was significantly decreased （（ 1.91 ±0.25） vs （3.08±0.31）, P〈0.01 ; （28.39±1.70）% vs （36.14±2.89）%, P〈0.05）. Compared with the group-housed animals, social isolation group showed significantly reduced GM volume in the hippocampus, amygdala,auditory cortex and retrosplenial cortex. Compared with the group-housed animals, the synaptophysin and PSD93 expression in the hippocampus （ synaptophysin： （ 0.61±0.03 ） vs （ 1.18±0.07）, P〈 0.05; PSD93 ： （0.54±0.03） vs （ 1.36±0.08）, P〈0.01 ） and the PSD93 expression in the amygdala （（0.97±0.08） vs （ 1.63±0.2）, P〈 0.01 ） were significantly decreased in social isolation group. Conclusion Social isolation can cause spatial memory deficits in adult rats. The atrophy of hippocampus and amygdala,together with the decreased synaptic plasticity in hippocampus and amygdala may be involved in spatial memory deficits in social isolation rats.

关 键 词：孤养 基于体素的形态学分析 突触相关蛋白 认知损害 

分 类 号：R749[医药卫生—神经病学与精神病学]