胰腺癌细胞纳米探针的磁共振荧光双模态成像及抗肿瘤效果观察  

Effects of QDs@Gd-RGD nano probe on MRI and fluorescent dual-modality imaging of pancreatic cancer cells and its anti-tumor role

作　　者：刘峰君[1] 叶雯 何欣源[1] 施裕新[1] 

机构地区：[1]上海市公共卫生临床中心,上海201508

出　　处：《中国临床医学》2016年第4期428-432,共5页Chinese Journal of Clinical Medicine

基　　金：上海市科学技术委员会上海市自然基金青年项目(13ZR1459400)~~

摘　　要：目的:探讨QDs@Gd-RGD纳米探针用于MIAⅡ胰腺癌细胞磁共振(MRI)荧光双模态成像的效果及其对胰腺癌细胞的抑制作用。方法:构建QDs@Gd-RGD纳米探针,将溶于磷酸盐缓冲液(PBS)的QDs@Gd-RGD纳米探针(QDs@GdRGD组)、Gd(Gd组)分别与MIAⅡ胰腺癌细胞共孵育1h,Control组不做处理,然后用荧光相差显微镜及1.5T MRI仪观察3组的成像特点。3组MIAⅡ胰腺癌细胞培养24h后,观察细胞形态、增殖、克隆、迁移、细胞周期、凋亡的变化。结果:荧光相差显微镜下观察发现,QDs@Gd-RGD纳米探针分布于MIAⅡ胰腺癌细胞膜,而Gd组及Control组细胞膜及细胞内未观察到荧光分布;1.5T MRI成像显示,QDs@Gd-RGD组信号强度明显高于Gd组、Control组(P<0.05)。QDs@Gd-RGD组MIAⅡ胰腺癌细胞失去典型铺路石样形态、细胞核碎裂、细胞质空泡化,细胞克隆能力明显下降,细胞增殖抑制,细胞迁移能力减弱,细胞周期明显被阻滞,细胞凋亡增加(P<0.05)。结论:QDs@Gd-RGD纳米探针用于MIAⅡ胰腺癌细胞MRI荧光双模态成像的效果较好;QDs@Gd-RGD纳米探针体外对MIAⅡ胰腺癌细胞有一定的杀伤作用。Objective：To investigate the effects of QDs@ Gd‐RGD nano probe on fluorescent and MRI dual‐modality imaging of M IA Ⅱ pancreatic cancer cells and its role in inhibiting pancreatic cancer cells .Methods：QDs@ Gd‐RGD nano probe was prepared .QDs@ Gd‐RGD nano probe （QDs@ Gd‐RGD group） and Gd （Gd group） were dissolved in phosphate buffer solution （PBS） and incubated with MIA Ⅱ pancreatic cancer cells for 1 h ,and no treatment was given to the control group .And then imaging characteristics of the 3 groups were observed by fluorescence phase contrast microscope and 1 .5 T MRI observation .After 3 groups of MIA Ⅱ pancreatic cancer cells were cultured for 24 h ,cell morphology ,proliferation , cloning ,migration ,cycle and apoptosis of MIA Ⅱ pancreatic cancer cells were observed .Results：Fluorescence phase contrast microscopy showed that the QDs@ Gd‐RGD nanoparticles were distributed in MIA Ⅱ pancreatic cancer cell membrane ,while no cell membrane and cell fluorescence distribution was observed in the Gd group and the control group .1 .5 T MRI imaging showed that the signal intensity of QDs@ Gd‐RGD group was significantly higher than that of the Gd group and the control group （P〈0 .05） .QDs@ Gd‐RGD group MIA Ⅱ pancreatic cancer cells lost the typical paving‐stone morphology ,nuclear fragmentation and cytoplasmic vacuolization took place , cell cloning ability decreased significantly , cell proliferation was inhibited ,cell migration ability decreased ,cell cycle was significantly blocked and cell apoptosis increased （P〈 0 .05） . Conclusions：QDs@ Gd‐RGD nano probe is effective in fluorescence and MRI dual modality imaging of MIA Ⅱ pancreatic cancer cells .Meanwhile ,QDs@ Gd‐RGD nano probe has a certain killing effect on MIA Ⅱ pancreatic cancer cells .

关 键 词：胰腺癌细胞 M RI荧光双模态成像 靶向诊断 抗肿瘤治疗 

分 类 号：R735.9[医药卫生—肿瘤]