卡巴胆碱对新生大鼠缺氧缺血脑损伤和炎症的影响  

Effect of Carbachol on brain damage and inflammation of neonatal rats after hypoxia - ischemia

作　　者：徐艳[1] 张丽华[1] 李雪梅[1] 孙颖[1] 郭津[1] 康贝贝[1] 

机构地区：[1]佳木斯大学儿童神经康复实验室,154003

出　　处：《中华实用儿科临床杂志》2016年第21期1650-1653,共4页Chinese Journal of Applied Clinical Pediatrics

基　　金：佳木斯大学重点项目（Sz2014-002）

摘　　要：目的研究卡巴胆碱对新生大鼠缺氧缺血脑损伤和3个脑区小胶质细胞活化的影响。方法将60只7日龄Wistar大鼠按随机数字表法随机分为干预组、对照组和假手术组，每组各20只。干预组和对照组采用Rice法制作缺氧缺血脑损伤模型，假手术组仅游离左颈总动脉。干预组在模型建立后及建立后24 h、48 h 3次皮下注射0.12 mg/kg卡巴胆碱，对照组同时注射同等剂量的9 g/L盐水，假手术组不做干预。每组分别在干预完成后第1天和第7天处死10只大鼠，处死的大鼠均行HE染色，观察各组大鼠的大脑损伤，免疫组织化学染色检查活化小胶质细胞数量。结果1.HE染色：假手术组脑组织外观正常，未见任何病理改变；干预组和对照组脑组织损伤明显，且2 d对照组损伤情况（第1天：79.60±3.31，第7天：74.40±5.98）均重于干预组（第1天：94.40±2.22，第7天：91.10±2.51）（P均〈0.01）。2.活化小胶质细胞情况：假手术组几乎无活化的小胶质细胞；对照组从第1天到第7天大脑皮层中活化小胶质细胞明显变多且持续存在（第1天：280.40±3.06，第7天：292.40±10.01，P〈0.05），而海马区和白质第7天活化小胶质细胞数量显著减少（P均〈0.05）；与对照组相比，第1天和第7天干预组各区域活化小胶质细胞数量明显减少，差异有统计学意义（P均〈0.05）。结论缺氧缺血致脑损伤可引起小胶质细胞活化；卡巴胆碱对新生大鼠缺氧缺血脑损伤有保护作用。Objective To study the effects of Carbachol on brain damage and inflammation after hypoxia - ischemia（HI） in neonatal rats. Methods Sixty of 7 - day Wistar rats were randomly divided into 3 groups ： intervention group, control group and sham operation group, with 20 rats per group. According to the Rice method, 40 HI models （i. e. intervention and control group） were established, and in the sham operation group, rats had an operation that only dissociated the left common carotid artery instead of HI. In the intervention group,the rats received a subcutaneous injection of Carbachol （0.12 mg/kg） after HI ,24 h and 48 h after HI. Meanwhile, the rats of control group received an e- quivalent volume of saline for 3 times. The rats were killed on day 1 and day 7 after injection and brain was checked damage were evaluated through HE staining and the number of microglias in 3 regions of brain was checked through im- munohistochemistry. Results （1） HE staining： the brain issues were normal in appearance and there was not any pathological changes in sham operation group. The brains were damaged both in intervention group （the first day： 94.40±2.22, the seventh day ：91.10±2.51 ）and control group （the first day ：79.60±3.31, the seventh day ：74.40 ±5.98）, and the damage degree was more serious in control group （ all P 〈 0.01 ）. （2） Microglial accumulation ： there was no microglial accumulation in sham operation group, but it was evident in the intervention group and control group （ P 〈 0.05 ）. In the control group, microglial accumulation in the cortex was persistent from day 1 to 7 （ the first day ： 280.40±3.06, the seventh day ：292.40±10.01, P 〈 0.05 ）, conversely, the number of microglia was attenuated in the hippocampus and white matter on day 7 （P 〈 0.05 ）. The number of microglia was significantly less in intervention group than that in the control group on day 1 and 7, and the difference was statistically significant （ P 〈 0.05 ）. Conclus

关 键 词：缺氧缺血 卡巴胆碱 炎症 小胶质细胞 

分 类 号：R742[医药卫生—神经病学与精神病学]