阿维A与干扰素对人皮肤T细胞淋巴瘤细胞株Hut78细胞增殖及白细胞介素15表达的影响  

Effects of acitretin and interferon on the proliferative activity of and interleukin-15 expression in a human cutaneous T-cell lymphoma cell line Hut78

作　　者：于凯[1] 王一宇[2] 金仙花[2] 李雪[2] 朱文静[2] 夏建新[2] Yu Kai Wang Yiyu Jin Xianhua Li Xue Zhu Wenjing Xia Jianxin(Department of Dermatology, Liaocheng People＇s Hospital, Liaocheng 252000, Shandong, China Department of Dermatology, The Second Hospital of Jilin University, Changchun 130041, China)

机构地区：[1]聊城市人民医院皮肤科,山东252000 [2]吉林大学第二医院皮肤科

出　　处：《中华皮肤科杂志》2016年第11期811-815,共5页Chinese Journal of Dermatology

基　　金：吉林省科技发展项目（20130413015GH）

摘　　要：目的：检测不同浓度阿维A、干扰素α（IFN-α）单独或联合应用对人皮肤T细胞淋巴瘤Hut78细胞株的增殖抑制作用及白细胞介素15（IL-15）表达的影响。方法将Hut78细胞分为空白对照组、阴性对照组、二甲基亚砜组（DMSO）和实验组，其中实验组分别用0.1~10μmol/L阿维A和5000~20000 IU/ml IFN-α单独或1.0μmol/L阿维A联合5000~20000 IU/ml IFN-α作用Hut78细胞，培养24、48、72 h后分别进行测定。CCK8法检测细胞增殖情况，ELISA检测药物处理后细胞的白细胞介素15（IL-15）表达情况。结果各浓度阿维A或联合作用组与DMSO组相比及IFN-α组与阴性对照组相比，Hut78细胞增殖和IL-15表达均受到明显抑制，抑制作用随浓度增加和时间延长而增强。重复测量方差分析显示，阿维A、IFN-α单独或联合作用不同时间，细胞增殖抑制率和IL-15表达差异均有统计学意义（P〈0.05），不同作用浓度之间差异亦有统计学意义（均P〈0.05），作用时间与药物浓度之间存在交互作用（均P〈0.05）。1.0μmol/L阿维A＋10000或20000 IU/ml IFN-α组与相应浓度药物单独作用组比较，细胞抑制率差异在24、48、72 h时均有统计学意义（P〈0.05）。1.0μmol/L阿维A＋5000 IU/ml IFN-α组在24、48、72 h时与5000 IU/ml IFN-α组相比，IL-15表达差异均有统计学意义（P〈0.05）；1.0μmol/L阿维A＋10000或20000 IU/ml IFN-α组与相应浓度药物单独作用组之间IL-15表达差异在24、48、72 h时均有统计学意义（P〈0.05）。结论阿维A和IFN-α均对Hut78细胞的增殖有抑制作用，均可下调Hut78细胞IL-15的表达，这种作用随着浓度的增加和时间的延长而增强，且两者联合用药优于单独用药。Objective To evaluate effects of acitretin and interferon-α（INF-α）alone or in combination on the proliferative activity of and interleukin-15 expression in human cutaneous T-cell lymphoma Hut78 cells. Methods Cultured Hut78 cells were divided into several groups, including blank control group, negative control group, dimethyl sulphoxide （DMSO） group and experimental groups. Cells in experimental groups were additionally classified into several subgroups to be treated with acitretin（0.1-10μmol/L, acitretin groups）or INF-α（5 000-20 000 IU/ml, INF-αgroups） alone, or the combination of 1.0 μmol/L acitretin and IFN-α at concentrations of 5 000- 20 000 IU/ml （combination groups）, for 24, 48 and 72 hours. Subsequently, cell counting kit 8（CCK8）assay was performed to assess the proliferative activity of Hut78 cells, and enzyme-linked immunosorbent assay（ELISA）to measure the expression of IL-15 in these cells. Results The proliferative activity of and IL-15 expression in Hut78 cells were both obviously suppressed in the acitretin groups and combination groups compared with the DMSO group, as well as in the INF-αgroups compared with the negative control group, and the inhibitory effects gradually increased with the increase in acitretin or INF-αconcentrations and treatment durations. As repeated measures analysis of variance revealed, there was a significant difference in both proliferation inhibition rates and IL-15 expression among different treatment durations and among different concentrations of acitretin or INF-α（all P〈0.05）, and there was an interaction effect between treatment durations and drug concentrations（all P〈0.05）. A significant difference was observed in both proliferation inhibition rates and IL-15 expression at 24, 48 and 72 hours when the 1.0-μmol/L acitretin＋ 10 000/20 000-IU/ml IFN-αgroup was compared with the 1.0-μmol/L acitretin group and 10 000/20 000 IU/ml IFN-αgroup（all P〈0.05）. There was also a significant difference in IL-15 expressi

关 键 词：淋巴瘤 T细胞 皮肤 阿维A 干扰素α 白介素15 细胞增殖 细胞系 肿瘤 Hut78细胞 

分 类 号：R739.5[医药卫生—肿瘤]