宁夏野生甘草中产甘草黄酮耐盐内生真菌分离及其DPPH自由基清除作用  

Separation of Licorice Flavonoids-Producing Salt-resistant Endophytic Fungi from Wild Glycyrrhiza uralensis Fisch Living in Ningxia District

作　　者：高晓娟[1,2,3] 吴秀丽[1,2] 吴玉玲[1] 陈靖[1,2,3] 

机构地区：[1]宁夏医科大学,药学院 [2]宁夏回药现代化工程技术研究中心 [3]宁夏医科大学回医药现代化省部共建教育部重点实验室,银川750004

出　　处：《天然产物研究与开发》2016年第10期1549-1556,共8页Natural Product Research and Development

摘　　要：采用组织分离法,在不含盐及含盐PDA培养基上分离、纯化内生菌,以甘草苷等8种甘草黄酮为对照品,分析发酵产物;DPPH自由基清除法测定清除率。共得到108株内生菌,TLC发现31株可能产甘草黄酮;HPLC-UV发现产甘草苷7株,产异甘草苷3株,产甘草素3株,产甘草查尔酮A3株,产刺甘草查尔酮1株,均为耐盐菌;产甘草苷、异甘草苷、甘草查尔酮A、刺甘草查尔酮的部分菌株有较强DPPH清除率;产甘草苷和异甘草苷的菌株8-5-Y-2活性最强,与甘草总黄酮相当,强于甘草查尔酮A。盐协迫得到产甘草黄酮,且具较强DPPH自由基清除活性的耐盐内生真菌,为资源替代奠定基础。The PDA medium containing different concentrations of NaCI was used to isolate the salt-resistant endophytic fungi from wild Glycyrriza uralensis. The extracts were analyzed by TLC and HPLC-UV methods to screen the licorice flavonoids-producing fungi. Totally 108 fungi were obtained and 31 strains were proved to possibly produce licorice flavonoids by TLC. Analyzed by a HPLC-UV method,7 strains of fungi were manifested to produce liquiritin and 3 strains produced isoliquiritin. 3 strains produced glycyrrhizin and 3 strains were observed to produce licochalcone A. Finally, the strain named 8-2-G-3 was found to produce echinatin. The DPPH free radical scavening assay results showed that some flavonoids-producing strains, such as liquiritin, isoliquiritin, licochalcone A, and echinatin, had strong antioxidant activi- ties. The strain 8-5-Y-2, which produced liquiritin and isoliquiritin, had a strong activity euqal to total licorice flavonoids while stronger than licochalcone A. Salt-resistant culture was beneficial to obtain the licorice flavonoids-producing saltresistant fungi which had strong DPPH free radical scavening activity.

关 键 词：甘草 耐盐内生真菌 甘草黄酮 HPLC-UV DPPH 

分 类 号：R932[医药卫生—生药学]