橄榄苦苷对大鼠心肌缺血再灌注损伤的保护作用  

Protective Effect of Oleuropein on Myocardial Ischemia-Reperfusion Injury in Rats

作　　者：王昱[1] 秦序[1] 

机构地区：[1]陇南师范高等专科学校生化系,成县742500

出　　处：《天然产物研究与开发》2016年第10期1643-1646,1519,共5页Natural Product Research and Development

基　　金：甘肃省自然科学基金(1107RJZK243);甘肃省高等学校科研资助项目(1128B-01)

摘　　要：为了探讨橄榄苦苷联合合心爽对大鼠心肌缺血再灌注损伤的影响及其保护作用机制,将50只健康大鼠分成假手术组、模型组、橄榄苦苷组、合心爽组、橄榄苦苷+合心爽组。经结扎冠脉左前降支制备心肌缺血再灌注大鼠模型,造模后药物处理7 d,用放射免疫法检测心肌肿瘤坏死因子α(tumor necrosis factor-α,TNF-α)、白细胞介素1(interleukin-1β,IL-1β)、白细胞介素10(interleukin-10,IL-10)含量,用比色法检测心肌超氧化物歧化酶(superoxide dismutase,SOD)、过氧化氢酶(catalase,CAT)活性及丙二醛(malondialdehyde,MDA)含量,用Western blot法检测心肌内皮素-1(ednothelin-1,ET-1)的表达。结果显示与假手术组相比,模型组心肌TNF-α、IL-1β、MDA的含量显著升高(P<0.01),IL-10、SOD、CAT水平均显著降低(P<0.01),心肌ET-1表达水平显著升高(P<0.01)。与模型组比较,经橄榄苦苷或合心爽治疗后,心肌TNF-α、IL-1β、MDA的含量显著降低(P<0.05,P<0.01),IL-10、SOD、CAT水平均显著升高(P<0.05,P<0.01),心肌ET-1表达水平显著降低(P<0.05,P<0.01)。橄榄苦苷和合心爽联合治疗后心肌缺血再灌注损伤的恢复更加显著。通过本研究可以看出橄榄苦苷和合心爽对小鼠心肌缺血再灌注损伤具有明显的保护作用,其机制可能与降低心肌内皮素-1表达、改善抗氧化酶活性和抑制炎症因子水平有关。The objective of this study was to explore the protective effect and the mechanism of oleuropein and dihiazem on myocardial ischemia-reperfusion injury in rats. Fifty health rats were divided into five groups, namely sham group, model group, oleuropein group,diltiazem group, oleurepein and diltiazem group. The model was established by the ligation of descending coronary artery in rats. After modeling, the rats were administrated with oleuropein, diltiazem, and oleuropein ＋ diltiazem for 7 d. The contents of tumor necrosis factor α （TNF-α） , interleukin-1β （IL-1β） and interleukin-10 （IL-10） were detected by radioimmunoassay. The activities of superoxide dismutase （SOD） and catalase （CAT） as well as the content of malondialdehyde （MDA） were measured by spectrophotometry. The changes of myocardial ednothelin-1 （ ET-1 ） expression in myoeardium were analyzed by Western blot assay. The results showed that compared to sham group, the contents of TNF-α, IL-1β and MDA were significantly increased, the levels of IL-10, SOD and CAT were significantly reduced,the myocardial ET-1 expression was significantly up-regulated in myocardium of model group mice （P 〈0.01 ）. Compared to model group with the treatments of oleuropein,dihiazem,and oleuropein ＋ dihiazem,the contents of TNF-α, IL-1β and MDA were significantly decreased, the levels of IL-10, SOD and CAT were significantly increased, the myocardial ET-1 expression was significantly down-regulated in myocardium of treatment group （P 〈 0. 05, P 〈0. 01 ）. Furthermore, the oleurepein ＋ diltiazem combined administration showed an even more significant positive effect. Based on these results,it was concluded that the treatments of oleuropein and diltiazem had a protective effect on myocardial ischemia-reperfusion injury.the mechanisms of which may depend on reducing myocardial ET-1 expression. improving antioxidant enzyme activity and inhibiting inflammatory response.

关 键 词：橄榄苦苷 心肌缺血再灌注损伤 合心爽 炎症因子 抗氧化能力 ET-1 

分 类 号：R285.5[医药卫生—中药学]