二重PCR快速检测中蜂囊状幼虫病病毒与东方蜜蜂微孢子虫方法的建立  

Establishment of duplex PCR rapid detection method for CSBV and Nosema ceranae in Apis cerana cerana

作　　者：林丽花[1] 黄伟峰[1] 陈琼艳 郭少娟[1] 赵翠霞[1] 雷珊珊[1] 苏松坤[1] 李继莲[2] 黄少康[1] 

机构地区：[1]福建农林大学蜂学学院,福州350002 [2]中国农业科学院蜜蜂研究所,北京100093

出　　处：《中国科技论文》2016年第18期2100-2104,共5页China Sciencepaper

基　　金：教育部留学回国人员基金资助项目(K4115005A);福建省对外合作项目(2016I0002);国家农业部"948"项目(2015-Z9)

摘　　要：中蜂囊状幼虫病和东方蜜蜂微孢子虫病是中蜂中常见的病害,对中蜂生产造成巨大危害,病原的早期快速检测是病害防控的重要手段。建立1种基于RNA提取的,快速、准确鉴定中蜂囊状幼虫病病毒(Chinese sacbrood virus,CSBV)和东方蜜蜂微孢子虫(Nosema ceranae,Nc)2种病原的二重PCR检测法。该方法同时检测CSBV和Nc的灵敏度可分别低至6与3个拷贝,且准确可靠;与两病原的单一PCR检测法灵敏度和精度一致,而二重PCR方法更加简便快速,为中蜂囊状幼虫病和东方蜜蜂微孢子虫病的早期快速诊断提供了重要的手段。Chinese sacbrood disease caused by Chinese sacbrood virus （CSBV） and nosemosis caused by Nosema ceranae （Nc） in Chinese honey bee （Apis cerana cerana） are common, and devastating to native beekeeping in China. Therefore, a quick and precise diagnosis of both pathogens at early stages is highly helpful for disease control. A duplex PCR method for rapid and precise detecting CSBV and Nc simultaneously was established based on RNA extraction. The lowest limits of simultaneous detection are 6 copies of CSBV and 3 copies of Nc, moreover, this method is precise and dependable. The sensitivity and precision of this method are similar to that of conventional single PCR method. The duplex PCR can serve as a simple, rapid, and precise method for early diagnosis of CSBV and Nc.

关 键 词：蜂学 中华蜜蜂 中蜂囊状幼虫病病毒 东方蜜蜂微孢子虫 二重PCR 

分 类 号：S895[农业科学—特种经济动物饲养]