三维细胞培养技术应用于肿瘤细胞的研究进展  

A review of three dimensional cell culture systems for tumor cells

作　　者：詹世淮 雷艳 黄梁浒 谭建明 

机构地区：[1]福建省移植生物学重点实验室,福州350025

出　　处：《中华细胞与干细胞杂志（电子版）》2016年第4期248-251,共4页Chinese Journal of Cell and Stem Cell（Electronic Edition）

基　　金：国家自然科学基金资助项目(81270431)

摘　　要：三维细胞培养是一种模拟细胞在体内微环境生长的新兴培养技术。在三维支架中细胞能以三维空间的方式生长,并以三维方式与周围的微环境交互作用,能充分体现肿瘤细胞的黏附、侵袭及远端转移过程,可作为动物模型和二维培养模型之间的桥梁。三维细胞培养技术广泛应用于肿瘤模型构建、肿瘤细胞生理学研究以及肿瘤耐药机制分析等的研究。3D cell culture is a new technology which can more accurately simulate the growth microenvironment of cells in vivo. Cells grown in three dimensional scaffolds interact with the surrounding microenvironment. 3D cell culture can fully reflect cell adhesion, invasion and metastasis, which is expected to bridge the gap between 2D culture and animal models. Here we review the current advances in cell physiology research, cancer drug resistance mechanism analysis and the construction of tumor model based on 3D cell culture.

关 键 词：三维 细胞培养技术 肿瘤细胞 药物 模型 结构 

分 类 号：R730.2[医药卫生—肿瘤]