不同栽培原料及配方对蛹虫草主要活性成分的影响  

Effects of Different Culture Raw Material and Formula on the Key Active Ingredients of Cordyceps militaris

作　　者：方华舟[1] 李先良[1] 

机构地区：[1]荆楚理工学院生物工程学院,湖北荆门448000

出　　处：《北方园艺》2016年第21期150-154,共5页Northern Horticulture

基　　金：湖北省教育厅重点科研资助项目(D20126101)

摘　　要：以大米为主要栽培基质,以常见的碳源氮源进行单因素试验,探索了不同碳源氮源对蛹虫草主要活性成分的影响及规律。结果表明:葡萄糖等寡糖类碳源及蛋白胨、蚕蛹粉等蛋白类氮源促进合成蛋白质、虫草素作用显著,葡萄糖、蛋白胨能力最强;柠檬酸铵、蛋白胨及果糖、蔗糖、葡萄糖促进虫草多糖合成,且柠檬酸铵作用显著;可溶性淀粉、果糖、蔗糖及柠檬酸铵、硝酸铵、蚕蛹粉等促进虫草酸合成,且柠檬酸铵、可溶性淀粉作用显著。以葡萄糖等寡糖、可溶性淀粉、蛋白胨或蚕蛹粉、柠檬酸铵等配方组合,可有效提高蛹虫草主要物质合成及主要活性成分含量。Different carbon sources and nitrogen sources were added to the rice medium to study their effect on the active ingredients of Cordyceps militaris.The results showed that such oligosaccharide carbon source as glucose,and nitrogen source as peptone and silkworm pupaprotein powder could markedly increase the content of protein and cordycepin of Cordyceps militaris.Glucose and peptone were the best for increasing the content.Ammonium cirate peptone,sucrose and glucose could promote the formation of the cordyceps polysaccharide,and ammonium cirate was the best.Starch,fructose,ammonium cirate ammonium nitrate and silkworm pupaprotein powder could prominently accelerate the synthesis of cordyceptic acid,the effects of ammonium cirate and starch were the most remarkable.These results indicated that good proportional addition of carbon source,such as oligosaccharide and starch,and nitrogen source such as peptone,ammonium citrate,silkworm pupaprotein powder could effectively co-increase the content of the key active ingredients of Cordyceps militaris.

关 键 词：蛹虫草 碳源 氮源 活性成分含量 机制 

分 类 号：S567.35[农业科学—中草药栽培]