羧甲基茯苓多糖对肠癌小鼠生命延长及对环磷酰胺的减毒作用  

Effect of Carboxymethyl Pachymaran on Life Extension and Attenuation of Cyclophosphamide-Induced Toxicity in CT26 Tumor-Bearing Mice

作　　者：王灿红 霍小位 何晓山[2] 刘冬羽[1] 李立勇[1] 曹丽[1] 

机构地区：[1]中国医学科学院药用植物研究所,北京100193 [2]云南中医学院中药学院,云南昆明650500

出　　处：《食品科学》2016年第21期229-233,共5页Food Science

基　　金：国家科技部“重大新药创制”科技重大专项(2012ZX09501001-004;2012ZX09301-002-001-026)

摘　　要：目的:研究羧甲基茯苓多糖(carboxymethyl pachymaran,CMP)对结肠癌(CT26)小鼠的生命延长及对环磷酰胺(cyclophosphamide,CTX)的减毒作用。方法:建立小鼠CT26皮下移植瘤模型,减毒增效实验分为:模型组、CTX组、CMP+CTX组;延长生命实验分为:模型组、CMP低、中、高剂量组。检测外周血白细胞(white blood cell,WBC)数、骨髓有核细胞(bone marrow nucleated cell,BMNC)数、肿瘤抑制率、脏器指数以及肿瘤组织中信号调节蛋白-α(signal?regulatory?protein-α,SIRP-α)和巨噬细胞表面标志物(F4/80)的表达,统计生命延长率和中位生存期。结果:CMP抑制CTX化疗所致的脾脏和胸腺指数的降低及WBC和BMNC数量的减少,拮抗肝脏指数升高,降低SIRP-α和F4/80的表达,并延长小鼠带瘤生存时间。结论:CMP明显减轻CTX的毒副作用,且具有调节肿瘤免疫相关蛋白表达和延长CT26小鼠带瘤生存时间的作用。Objective：To investigate the effect of carboxymethyi pachymaran（CMP） on life extension of colon tumor 26（CT26）-bearing mice and mitigating the side effects caused by chemotherapy with cyclophosphamide（CTX）.Methods：CT26 tumor-bearing mouse models were established by subcutaneous transplantation into mice,and they were randomly divided into model,CTX and CTX ＋ CMP groups for toxicity test.The model,and low-,medium- and high-dose CMP,groups were used for life extension test.Peripheral white blood cells（WBC）,bone marrow nucleated cells（BMNC）,percentage tumor inhibition,and visceral organ indexes were tested by biochemical assays.The expression of signal regulating protein alpha（SIRP-α） and macrophage surface marker（F4/80） in tumor tissues were detected through immunohistochemistry.Meanwhile,life extension rate and median survival period were observed.Results：CMP could increase the lower spleen index and thymus index,and the decreased levels of WBC and BMNC induced by CTX,antagonize the increase in liver index,and reduce the expression of SIRP-α and F4/80 in tumor tissues.In addition,CMP also could extend the survival time of CT26 tumor-bearing mice.Conclusion：CMP can obviously reduce the side effects of CTX,regulate the expression of tumor immune-related proteins,and prolong the survival time of CT26 tumor-bearing mice.

关 键 词：羧甲基茯苓多糖 结肠癌 免疫调节 环磷酰胺 减毒作用 生命延长 

分 类 号：R285.5[医药卫生—中药学]