正常兔眼玻璃体微泡注射联合超声辐照干预中最适超声参数的筛选  

Screening of optimal ultrasound parameters after intravitreal injection of microbubble in normal rabbit eyes

作　　者：申晓丽[1] 李强[2] 黄丽娜[1] 赵军[1] 樊宁[1] 

机构地区：[1]暨南大学附属深圳市眼科医院 深圳市眼病研究所 深圳市眼科学重点实验室,深圳518040 [2]暨南大学附属深圳市人民医院眼科,深圳518000

出　　处：《中华实验眼科杂志》2016年第11期983-988,共6页Chinese Journal Of Experimental Ophthalmology

基　　金：国家自然科学基金面上项目（81170840）;广东省医学科学技术研究基金项目（A2015315）;深圳市创新委基础研究项目（JCYJ20120831154554508）

摘　　要：背景 微泡可作为药物和基因的转染载体,经超声照射后转染效率提高,从而在多种眼科疾病的治疗方面发挥重要作用.然而,不同超声辐照强度发挥的生物学效应有所不同. 目的 筛选适用于正常兔视网膜组织的超声辐照强度和时间,为超声联合微泡治疗眼底疾病的研究提供实验依据.方法 选取18只清洁级新西兰大白兔,按照随机数字表法分为空白对照组、单纯超声辐照组、单纯玻璃体腔注射组及玻璃体腔注射＋低、中、高强超声辐照组.空白对照组未给予任何处理,单纯超声辐照组和单纯玻璃体腔注射组分别给予2.0 W/cm^2的超声强度照射实验眼60 s或单纯玻璃体腔注射0.1ml微泡,玻璃体腔注射＋低、中、高强超声辐照组兔眼在玻璃体腔注射0.1ml微泡后分别用0.5、1.0和2.0 W/cm^2超声强度照射实验眼60 s.各组于术前、术后即刻、术后第1天、第7天分别行眼前节照相、直接检眼镜检查和彩色眼底照相观察眼前节和眼底情况,于术后第7天处死实验兔并分离视网膜,行苏木精-伊红染色,光学显微镜下观察实验眼视网膜形态学改变,透射电子显微镜观察兔视网膜超微结构变化.结果 空白对照组、单纯超声辐照组、单纯玻璃体腔注射组及玻璃体腔注射＋低、中、高强超声辐照组兔手术前后不同时间点眼前后节均未见明显异常.术后7d,光学显微镜下可见空白对照组、单纯超声辐照组、单纯玻璃体腔注射组和玻璃体腔注射＋低超声辐照组兔10层视网膜组织结构清晰,未见炎症、变性及细胞坏死等改变,而玻璃体腔注射＋中强超声辐照组和玻璃体腔注射＋高强超声辐照组兔眼视网膜各层组织结构疏松,视细胞层排列紊乱,内核层和外核层不同程度变薄,视网膜神经节细胞（RGCs）数量减少,可见细胞的空泡样变性.透射电子显微镜下可见空白对照组、单纯超声辐照组、单纯玻璃体腔注射Background Ultrasound microbubbles play important effects on delivery of drug or gene,which can significantly improve the transfection efficiency of drug or gene after ultrasonic irradiation.However,different ultrasonic irradiation parameters have different biological effects on different cells.Objectives This study was to investigate the effects of ultrasound microbubble on normal rabbit retina in different ultrasound intensities and irradiation time and provide the experimental basis for the treating study of fundus diseases with ultrasound microbubble.Methods Eighteen clean New Zealand albino rabbits were randomized into blank control group,simple ultrasound radiation group,simple intraocular injection group and intraocular injection＋low,moderate and high intensity ultrasound groups.No intervention was performed in the blank control group.Only 2.0 W/cm^2 ultrasound radiated for 60 seconds or 0.1 ml microbubbles were injected in vitreous cavity respectively in the simple ultrasound radiation group or simple intraocular injection group.The ultrasound with the intensity of 0.5,1.0 and 2.0 W/cm^2 radiated the eyes with intraocular injection of microbubbles for 60 seconds in the intraocular injection＋low,moderate and high intensity ultrasound groups,respectively.The photography of ocular anterior segment,direct ophthalmoscopy and color fundus photography were performed before operation,immediately after operation and 1 day,7 days after operation.The retinas were isolated in the seventh day and hematoxylin ＆ eosin staining was carried out for the evaluation of retinal morphology.The ultrastructure of the retinas was examined by transmission electron microscope.This study protocol was approved by Ethic Committee of Experimental Animals Use and Care in Shenzhen Eye Hospital.Results There were not abnomal findings by slit lamp microscope and ophthalmoscope in the eyes of various groups at different time points.In the intraocular injection＋moderate and high intensity ultrasound groups,the loosening of retinal la

关 键 词：视网膜 超声 微泡 超声处理 玻璃体腔注射 超声参数 兔 

分 类 号：R77[医药卫生—眼科]