基于生物传感器的发酵过程在线分析系统研制  

Development of biosensors based online analysis system for fermentation process

作　　者：朱思荣[1] 周万里[1] 毕春元[1] 张利群[1] 杨艳[1] 张金玲[1] 赵晓华[1] 

机构地区：[1]山东省科学院生物研究所,山东省生物传感器重点实验室,山东济南250014

出　　处：《山东科学》2016年第5期90-97,110,共9页Shandong Science

基　　金：国家高技术研究发展计划(863计划)(2015AA021005)

摘　　要：基于发酵过程自动优化控制的需求，研制了发酵过程生物传感器在线自动取样分析系统。给出了在线分析系统的分析原理及其结构组成，详细介绍了自动取样、样品稀释及生物传感器信号处理的方法。系统可以实现对葡萄糖、乳酸和谷氨酸等重要生化参数的自动取样分析，并把分析结果通过485接口或4～20mA模拟接口发送到发酵控制器，为发酵控制提供生化分析数据。用葡萄糖标准样品作为发酵液对实验样机进行了测试，结果表明在线分析系统具有较高的分析精度，可以满足发酵过程分析的需求。We developed a biosensors based online automatic sampling and analysis system in fermentation process for the requirements of automatic optimization control in fermentation process. We presented its analytical principle and structure components and detailed its automatic sampling, sample dilution and biosensor signal processing. It can automatically analyze such important biochemical parameters as glucose, lactic acid and glutamic acid, and send analytical results to fermentation controller through a 485 interface or a 4- 20 mA analog interface. It can therefore provide biochemical analytical data for fermentation control. We further tested experiment prototype with glucose standard sample as fermentation broth. Results show that it has higher analytical accuracy and can satisfy the requirements of fermentation process analysis.

关 键 词：生物传感器 自动分析仪 在线分析 葡萄糖分析 乳酸分析 发酵罐自动取样 

分 类 号：Q815[生物学—生物工程] TP216[自动化与计算机技术—检测技术与自动化装置]