沉默FOXC1基因对鼻咽癌细胞系5-8F凋亡和周期的影响  

Impact of silenced FOXC1 gene on apoptosis and cell cycle of nasopharyngeal carcinoma cell line 5-8F

作　　者：易世江[1] 陈柏林[1] 申一鸣[1] 陈莹[1] 刘鹏[1] 雷迅[1] 

机构地区：[1]桂林医学院附属医院耳鼻咽喉头颈外科,541001

出　　处：《实用医学杂志》2016年第21期3517-3520,共4页The Journal of Practical Medicine

基　　金：广西桂林市科技局科技攻关项目(编号:20140505-3);广西教育厅课题(编号:200911MS168)

摘　　要：目的:探讨沉默叉头框C1(forkhead boxC1,FOXC1)基因对鼻咽癌细胞系5-8F凋亡和细胞周期的影响。方法:利用si RNA技术在5-8F细胞中沉默FOXC1基因,通过流式细胞仪检测细胞凋亡和周期的变化,应用Western blot方法检测相关蛋白Bcl-2、CyclinD1的表达。结果:FOXC1基因沉默后5-8F细胞的凋亡率明显增高,且Bcl-2蛋白表达减弱(P<0.05),处于G1期的细胞数减少,处于S期的细胞数增多,同时降低了CyclinD1蛋白的表达(P<0.05)。结论:沉默FOXC1基因可能通过下调Bcl-2表达促进5-8F细胞凋亡,又可能通过调节CyclinD1的表达影响5-8F细胞周期分布,这说明FOXC1在鼻咽癌进展中的作用值得进一步研究。Objective To explore the impacts of silenced FOXC1 gene on the apoptosis and cell cycle of NPC cell lines 5-8F. Methods siRNA was used to silence FOXC1 gene in 5-8F ceils. The apoptosis and cell cycle of 5-8F cells were then detected by flow cytometry, and the protein expressions of Bcl-2 and CyclinD1 were detected by Western blot. Results The apoptotic rate was increased significantly, the cell cycle distribution in the G1 and S phase were changed, and the protein expressions of Bcl-2 and CyclinD1 were decreased in 5-8F cells after FOXC1 was silenced （P〈0.05）. Conclusions Silenced FOXC1 gene may induce the apoptosis of 5-8F cell line by regulating the expression of Bcl-2, and it may also change the cell cycle distribution of 5-8F cell line through adjusting the expression of CyclinD1, showing the role of FOXC1 in development of nasopharyngeal carcinoma is worth further researching.

关 键 词：鼻咽癌 FOXC1 基因沉默 BCL-2 CYCLIND1 

分 类 号：R739.63[医药卫生—肿瘤]