MTHFR基因多态性与广州市老年人缺血性脑卒中的相关性  

Correlation between the polymorphism of methylenetetrahydrofolate reductase gene and ischemic stroke in old people in Guangzhou

作　　者：于小琪[1] 秦琴保[1] 王双艳[1] 雷秀霞[1] 刘丰[1] 

机构地区：[1]广州市第一人民医院老年病科,510180

出　　处：《实用医学杂志》2016年第21期3540-3542,共3页The Journal of Practical Medicine

基　　金：广州市卫生局医药卫生科技项目(编号:20151A010012)

摘　　要：目的:探讨亚甲基四氢叶酸还原酶(MTHFR)基因C677T位点多态性与广州市老年人缺血性脑卒中发生的关系。方法:选取2015年12月至2016年5月在广州市第一人民医院老年病科住院及体检中年龄>75岁非急性期缺血性脑卒中患者139例为病例组,并行TOAST分型,另选同期健康汉族广州市老年人135例为对照组。采用聚合酶链反应-限制片段长度多态性法检测MTHFR基因C677T位点。结果:两组患者MTHFR基因C677T位点3种基因型分布比较,差异有统计学意义(P<0.05);两组C、T等位基因分布频率差异也有统计学意义(P<0.05)。病例组以CC基因型为主(43.2%),对照组以CT因型为主(48.9%)。病例组TT基因型频率(18.7%)较高,其中CE的TT基因型频率(27.2%)较高。结论 :MTHFR基因C677T位点TT基因型是广州市老年人缺血性脑卒中的危险因素。Objective To explore the relationship between the Polymorphism of Methylenetetrahydrofolate Reductase Gene and ischemic strokein old People in Guangzhou. Methods One hundred and thirty-nine patients aged more than 75 years old with non-acute ischemic stroke were admitted into the Department of Geriatrics, Guangzhou First People＇s Hospital from December 2015 to May 2016. The polymerase chain reaction- restriction fragment length polymorphism （PCR-RFLP） method was used to analyze the MTHFRC677T genepolymorphism. Results Significant differences （P 〈 0.05） were noted in the CC, CT, TT genotype distribution of the MTHFRC677T gene between the two groups; The case group was higher （P 〈 0.05） than the control group in the C, T allele frequency. CC genotype was dominant in the case group （43.2%）, CT genotype was the dominant in the control group（48.9%）. Conclusion Tr genotype of MTHFRC677T gene is an important risk factor for old people in Guangzhou with ischemic stroke.

关 键 词：缺血性脑卒中 亚甲基四氢叶酸还原酶 基因多态性 广州市老年人 TOAST分型 

分 类 号：R743.3[医药卫生—神经病学与精神病学]