VCA+EA多肽片段联合包被ELISA对血液EBV IgA检测效果的研究  

Evaluation of double peptides of VCA and EA coated ELISA test in detection of serum EBV IgA

作　　者：陈明星 廖秀梅 刘万里[2] 

机构地区：[1]广东省龙川县人民医院,517300 [2]中山大学附属肿瘤医院,广东广州510060

出　　处：《国际检验医学杂志》2016年第21期3024-3026,共3页International Journal of Laboratory Medicine

摘　　要：目的探讨中山大学附属肿瘤医院研制的病毒壳抗原(VCA)+早期抗原(EA)多肽片段联合包被聚苯乙烯微孔板酶联免疫吸附测定(ELISA)方法对血液中Epstein-Barr病毒(EBV)免疫球蛋白A(IgA)的检测效果,并将其与进口筛选试剂盒进行比较,最终对VCA+EA多肽片段联合包被ELISA试剂盒性能进行评价。方法收集鼻咽癌患者血清86例,健康人群血清100例,用ELISA检测血清中IgA的OD值,观察该方法的最低检测限、线性范围、精密度、回收率,同时采用进口的Epstein-Barr病毒持续表达核抗原(EBNA1)+VCA-p18抗原包被ELISA方法进行检测,观察其临床性能。结果 VCA+EA多肽片段联合包被ELISA方法最低检测限为(0.003 4±0.002 6),血清稀释100倍数(及以上),其水平与吸光度呈线性关系,阴性标本、弱阳性标本、阳性标本IgA水平的日内变异系数为3.97%、9.76%、9.23%,日间变异变异系数分别为7.56%、10.20%、7.67%。VCA+EA多肽片段联合包被试剂盒与EBNA1+VCA-p18抗原包被试剂盒比较,相关系数为0.926 6;检测鼻咽癌敏感性为88.90%,特异性为77.80%,阳性预测值为80.00%,阴性预测值为87.50%。结论 VCA+EA多肽片段联合检测鼻咽癌有较高敏感性,重复性较好,阴性结果预测较可靠,可作为临床筛查鼻咽癌的新方法。Objective To study the value of double peptides of VCA and EA coated ELISA test（EBNA1＋ EB-VCA-p18）in serological diagnosis of IgA of Epstein-Barr Viruses（EBV）and to compare the effect with import screening kit.Methods By synthetic peptides EBNA1 and EB-VCA-P18 to establish ELISA assay,serum samples from 86 cases with nasopharyngeal carcinoma and 100 healthy people were collected.And the OD value of IgA was detected.The lowest detectable limit,linearity,precision and recovery were observed.The import ELISA kit of EBV VCA-p18/IgA was used as comparison.Results The lowest detectable limit of this mothed was（0.003 4±0.002 6）after the serum was diluted above 100 times.The level of IgA and absorbance had linear correlation.Daily variation coefficients of the level of IgA in negative samples,weakly positive samples and positive samples were 3.97%,9.76% and 9.23%respectively.Between-day variation coefficients were 7.56%,10.20% and 7.67%respectively.The double peptides of VCA＋EA coated ELISA test was positively correlated with EBNA1＋EB-VCA-p18 coated ELISA test（r=0.926）.The sensitivity,specificity,positive predictive value,negative predictive value of the test were 88.90%,77.80%,80.00% and 87.50%respectively.Conclusion The double peptides of VCA＋EA coated ELISA test has good sensitivity and repeatability,and negative predictive results are reliable,which could be used as a new way to screen nasopharyngeal carcinoma.

关 键 词：鼻咽癌 EPSTEIN-BARR病毒 免疫球蛋白A 

分 类 号：R739.63[医药卫生—肿瘤] R730.43[医药卫生—临床医学]