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作 者:邓培渊[1] 袁伟[2] 李玉华[1] 王林青[1] 李长看[1]
机构地区:[1]郑州师范学院生物物种资源研究中心,河南郑州450044 [2]华北水利水电大学环境与市政工程学院,河南郑州450045
出 处:《华北农学报》2016年第5期233-238,共6页Acta Agriculturae Boreali-Sinica
基 金:河南省科技攻关重点项目(132102110175)
摘 要:为了研究触角结合蛋白(Antennal binding proteins,ABPs)的生理功能,利用RT-PCR方法克隆了小菜蛾Pxyl ABP基因,并在大肠杆菌中表达,纯化的重组蛋白通过荧光竞争结合试验测定其与29种化合物的结合特性,并运用Auto Dock 4.2进行Pxyl ABP蛋白和化合物的分子对接。结果表明,Pxyl ABP与异硫氰酸丙烯酯、己醛、己烷、2-己酮、吲哚和β-紫罗兰酮结合,解离常数分别为0.91,1.81,1.17,2.71,2.74,14.95μmol/L;分子对接显示,Tyr100与异硫氰酸丙烯酯形成1个氢键。说明Pxyl ABP参与了小菜蛾识别十字花科蔬菜和虫害诱导的植物挥发物的生理过程。Antennal binding proteins are soluble acidic proteins in insect antenna. It is an important way to clarify ABP physiological function by analysising the binding pattern. The Pxyl ABP gene of Plutella xylostella was cloned using reverse transcription PCR and expressed in Escherichia coli. The specificity of Pxyl ABP binding with 29 compounds was tested through fluorescence competitive binding assay,and the molecular docking of Pxyl ABP with compounds were carried out using Auto Dock 4. 2. The results showed that Pxyl ABP protein had binding ability to Allylisothiocyanate,Hexane,Hexanal,Indole,2-hexanone and β-Lonone,with the dissociation constants of 0. 91,1. 81,1. 17,2. 71,2. 74 and 14. 95 μmol/L,respcetively; The molecular docking results showed that one hydrogen bond generated between Tyr100 and Allyl-isothiocyanate. These data suggested that Pxyl ABP might be involved in the physiological process that discriminate the cruciferous vegetables and herbivore-induced plant volatiles in Plutella xylostella.
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