神农架川金丝猴投食群TRIM5 alpha基因的变异及其进化分析  

Variation and Evolution Analysis of TRIM5 alpha in Golden Snub-nosed Monkey Provisioned Group in Shennongjia National Natural Reserve

作　　者：张杜[1] 郭雪[1] 杨万吉 刘宏伟[1] 梁伊丽[1] 刘学端[1] 

机构地区：[1]中南大学资源加工与生物工程学院,湖南长沙410038 [2]湖北神农架金丝猴保育生物学湖北省重点实验室,湖北神农架442421

出　　处：《现代生物医学进展》2016年第28期5405-5409,5530,共6页Progress in Modern Biomedicine

基　　金：国家"十二五"科技支撑计划项目(2013BAD03B02)

摘　　要：目的:本研究探索神农架大龙潭川金丝猴TRIM5alpha蛋白序列的特征、变异、该基因进化特点以及该基因的遗传多样性,对金丝猴的保护和研究具有重要的意义。方法:从神农架川金丝猴投食群随机选择41个个体,采集毛发并提取基因组DNA,设计引物,对其TRIM5 alpha基因编码序列进行PCR扩增和测序,最后对序列进行比对和分析。结果:在神农架川金丝猴投食群41个个体中,TRIM5alpha基因不存在与Cyp A假基因序列融合的现象。与恒河猴序列不同而与人类相似的是,川金丝猴中没有发现TFP339-341型,而只发现△△Q339-341型。41个个体TRIM5alpha蛋白序列没发现任何非同义突变位点,显示该基因在该种群中具有很低的遗传多样性。编码序列的进化分析表明,Coiled-coil结构域和SPRY结构域在进化中经历了明显的正向选择,Ring结构域和B-box结构域则经历了纯化选择。结论:川金丝猴TRIM5 alpha基因存在很多独特变异位点,且经历了明显的正向选择。对投食群的研究表明该基因编码序列在该种群中多样性很低,这从侧面反映了该总群较低的遗传多样性。针对这种情况,建议在种群复壮的工作中,适当引入一批有效的建群者。Objective： In this investigation, we explore the sequence characteristics, mutation, gene evolution characteristics and the genetic diversity of TRIM5 alpha sequence in golden snub-nosed monkey provisioned group in Shennongjia National Natural Reserve. Methods： We selected 41 individuals randomly from the population, collected hair samples and extracted the genomic DNA. Then we designed the primers for PCR amplification taking rhesus monkeys TRIM5 alpha gene sequence as a reference and the products was sequenced. Finally, we alignment and analysis the sequence. Results： We did not detected a gene fusion phenomenon of TRIM5alpha and CypA pseudogene sequences in the 41 individuals. Compared with rhesus monkeys protein sequences, there is no genotype TFP339-341 was found, there is only genotype △△Q339-341 instead, which is similar to the TRIM5 alpha sequence of human. Sequence alignment of TRIM5alpha protein did not detected any non-synonymous mutations, showed that the gene has very low genetic diversity in the population. The analysis for the evolution of coding sequence of the gene shows that the Coiled-coil domain and the SPRY domain have been experiencing positive selection obviously in the evolution, the Ring domain and B-box domain have been experiencing purification selection. Conclusions： There is much unique mutation loci in the golden snub-nosed monkey TRIM5 alpha gene and this gene experienced obvious positive selection in the evolution. Studies on TRIM5 alpha in Golden Snub-nosed Monkey Provisioned Group in Shennongjia National Natural Reserve have shown that the gene coding sequence in the population diversity is rather low, suggested in fellowing work of population rejuvenation, the group needs to recruit suitable individuals to adapt the environment.

关 键 词：川金丝猴 抗病毒 TRIM5 ALPHA 变异 进化 

分 类 号：Q31[生物学—遗传学] S865.16[农业科学—野生动物驯养]