第二代测序技术的发展及应用  

Development and Application of Second- Generation Sequencing Technology

作　　者：张丁予[1] 章婷曦[1,2,3] 王国祥[1,2,3] 

机构地区：[1]南京师范大学地理科学学院,江苏南京210023 [2]江苏省地理信息资源开发与利用协同创新中心,江苏南京210023 [3]江苏省物质循环与污染控制重点实验室,江苏南京210023

出　　处：《环境科学与技术》2016年第9期96-102,共7页Environmental Science & Technology

基　　金：国家自然科学基金项目(41303058;41573061);江苏高校优势学科建设工程资助项目;江苏省太湖水污染治理专项(TH2014402)

摘　　要：基于双脱氧核苷酸末端终止测序法的第一代测序技术,由于其通量低、成本高的缺点,逐步被第二代测序技术取代。基于高通量测序的第二代测序技术由于其通量大、可定量、成本低廉的优点,在各个领域方面的研究越来越广泛。该文首先详细介绍了以454测序、Solexa测序和SOLiD测序为代表的第二代高通量测序技术的原理、方法,并将以这3种测序方法为基础构建的3种测序平台的优缺点、适用研究对象、测序精确度等进行了对比总结,然后对第二代测序技术目前在土壤、沉积物、人体、海洋和污水方面等领域的应用情况进行了介绍。最后,对第三代测序技术的发展作了相应展望。The second-generation sequencing technology based on the high-throughput sequencing is with the advantages of high-throughput and low cost compared with the first-generation sequencing technology based on the dideoxy chain termination method. Therefore, the second-generation sequencing technology is more widely used in various areas. This paper introduces the working principles and methods of the second-generation sequencing technologies which include 454 sequencing technology, Solexa sequencing technology and SOLID sequencing technology. Furthermore, applications of second-generation sequencing technology in soil, sediment, the human body, marine and sewage are reviewed. Finally, the prospects of the third-generation sequencing technology are discussed.

关 键 词：第二代测序技术 土壤 沉积物 人体 海洋 污水 

分 类 号：X172[环境科学与工程—环境科学]