生长激素释放肽对乳腺癌耐药蛋白表达的影响及意义  

Effect of Ghrelin on the Expression of Breast Cancer Resistance Protein （BCRP） in Breast Cancer Cells

作　　者：曲文志[1] 李子豪[1] 张云微 金光华[1] 杨蕾[1] 涂巍[1] 

机构地区：[1]中国医科大学附属第四医院,辽宁沈阳110032

出　　处：《医学临床研究》2016年第10期1880-1882,共3页Journal of Clinical Research

基　　金：辽宁省科学技术计划项目（编号：2013225303）

摘　　要：[目的]探讨生长激素释放肽（ghrelin）对乳腺癌耐药蛋白（BCRP）表达的影响。[方法]培养人乳腺癌细胞MDA—M＆231细胞，分别加入ghrelin、多柔比星、ghrelin联合多柔比星，采用tunnel方法检测细胞凋亡，western-blot方法检测细胞丝裂原活化蛋白激酶P38（P38）、磷酸化P38（p-P38）、BCRP的表达并比较。[结果]tunnel法检测显示人乳腺癌细胞MDA-MB-231在ghrelin的干预下增殖，而凋亡率在多柔比星的干预下显著降低，ghre—lin联合多柔比星能够抑制多柔比星对人乳腺癌细胞MDA-MB-231的杀伤作用（P〈0．001）。ghrelin干预组P38、p-P38蛋白表达增加、且BCRP蛋白表达明显上升；多柔比星干预组P38、p-P38蛋白表达下降；ghrelin联合多柔比星组较多柔比星组P38、p—P38蛋白表达增加，且BCRP蛋白表达明显上升（P〈0．05）。[结论]ghrelin激活P38MAPK信号通路促进乳腺癌细胞BCRP表达增加从而抑制多柔比星诱导乳腺癌细胞凋亡。[Objective] To study the effects of ghrelin on the expression of breast cancer resistance protein（BCRP） in breast cancer cell line. [Methods]Human breast cancer cell line MDA-MB-231 were cultured and treated with ghrelin, doxoruhiein, and ghrelin combined with doxoruhiein. The tunnel method was used to detect cell apoptosis. The Western- blot method was used to detect p-P38, P38, and BCRP protein levels. [Results]Tunnel assay showed that under the addition of ghrelin, the human breast cancer cell MDA-MB-231 proliferated and the apoptosis rate was significantly lower than when doxorubiein was used. Ghrelin combined with doxorubicin inhibits the killing effect of doxorubicin on the human breast cancer cell line MDA-MB-231（ P 〈0.001）. The P38 and p-P38 protein levels increased in the Ghrelin Group, and the protein expression of BCRP increased significantly as welt. The P38 and p-P38 protein levels and BCRP protein expression decreased in the Ghrelin combined with doxorubicin group, but the decrease was less compared to the doxorubicin group （ P〈0.05）. [Conclusion]The P38MAPK signaling pathway activated by ghrelin promotes breast cancer cell BCRP expression, inhibiting the killing effects of doxorubicin-induced apoptosis of breast cancer cells.

关 键 词：生长激素释放激素 肽类 乳腺肿瘤/药物疗法 药物耐受性 肿瘤细胞 培养的 

分 类 号：R737.9[医药卫生—肿瘤]