吡那地尔对术后持续性疼痛大鼠脊髓JNK/MCP-1释放的影响  

Effect of pinacidil on JNK/MCP-1 expression in spinal cord of a rat model of persistent postoperative pain

作　　者：朱翔[1] 曹苏[2] 秦毅彬[2] 佘庆[2] 丁晶晶[2] 杨建平[1] 

机构地区：[1]苏州大学附属第一医院麻醉科,江苏苏州215006 [2]南通大学附属医院麻醉科,江苏南通226001

出　　处：《东南大学学报（医学版）》2016年第5期678-682,共5页Journal of Southeast University(Medical Science Edition)

基　　金：国家自然科学基金资助项目(81400915);南通大学附属医院转化医学基地科研项目(TDFzh2014013)

摘　　要：目的:观察吡那地尔(pinacidil)对术后持续性疼痛大鼠脊髓JNK/MCP-1释放的影响。方法:随机将35只大鼠分为正常组(Control组)、假手术组(Sham组)、切割+牵拉皮肤肌肉组(SMIR组)、溶剂+SMIR组(Vehicle组)、吡那地尔+SMIR(Pina组)、吡那地尔+格列苯脲+SMIR组(Pina+Gli组)、格列苯脲+SMIR组(Gli组)等7组。检测各组机械刺激缩足反射痛阈值(MWT),Western blot/ELISA法检测脊髓JNK/MCP-1蛋白表达。结果:(1)与Sham组相比,SMIR组MWT在术后3 d开始降低(P<0.01),并持续21 d(P<0.001)以上;(2)SMIR术后持续显著上调脊髓JNK/MCP-1的表达(P<0.01);(3)鞘内注射吡那地尔显著下调术后SMIR诱导的脊髓JNK/MCP-1表达(P<0.01)和上调MWT(P<0.01),格列苯脲能阻断吡那地尔所引起的上述变化(P<0.05)。结论:吡那地尔能抑制切割及牵拉皮肤肌肉所致的术后持续性疼痛,其机制可能与抑制JNK/MCP-1的上调有关。Objective： To observe the effect of pinacidil on JNK/MCP-1 expression in spinal cord of a rat model of persistent postoperative pain. Methods： SD rats were divided into 7 groups randomly： control group（ Control group）,sham operation group（ Sham group）,skin / muscle incision and retraction group（ SMIR group）,vehicle＋ SMIR group（ Vehicle group）,pinacidil ＋ SMIR（ Pina group）,pinacidil ＋ glibenclamide ＋ SMIR group（ Pina＋ Gli group） and glibenclamide ＋ SMIR group（ Gli group）. The mechanical withdrawal threshold（ MWT） of the left hind paw of rats in different group was measured at different time points after the surgery. Western blot / ELISA were used to measure the protein expression level of JNK / MCP-1. Results：（ 1） Compared with Sham group,MWT in SMIR group was reduced significantly from 3 d after the operation（ P〈0. 01）,and lasted for more than 21 d（ P〈0. 001）.（ 2） The protein expression of JNK/MCP-1 were significantly increased after SMIR as compared with the control group（ P〈0. 01）.（ 3） Intrathecal injection of pinacidil attenuated the express of JNK / MCP-1 in the spinal cord（ P〈0. 01） and up-regulated the MWT（ P〈0. 01）,which could be blocked by glibenclamide（ P〈0. 05）. Conclusion： Pinacidil inhibits the persistent postoperative pain caused by SMIR. The mechanisms may be partly due to the inhibition of JNK / MCP-1 upregulation.

关 键 词：术后持续性疼痛 吡那地尔 JNK/MCP-1 脊髓 大鼠 

分 类 号：R-332[医药卫生] R972.4