植物乳杆菌Lp基因表达时内参基因的选择  

Reference Gene Selection of Gene Expression in Lactobacillus Plantarum Lp

作　　者：樊磊[1] 于长青[1] 姚笛[1] 张明玉[1] 李琳[1] 

机构地区：[1]黑龙江八一农垦大学食品学院,大庆163319

出　　处：《黑龙江八一农垦大学学报》2016年第5期51-55,67,共6页journal of heilongjiang bayi agricultural university

基　　金：黑龙江省自然基金重点项目资助(ZD201207);黑龙江省博士后专项经费资助(LBH-Q13133)

摘　　要：实验以植物乳杆菌Lp为实验材料,用荧光定量PCR技术分析16S rRNA、Glyceraldehyde-3-phosphate dehydrogenas、L-lactate dehydrogenase以及rec A protein四个候选内参基因的表达情况。应用两种软件ge Norm和Norm Finder程序对实验结果进行分析。实验结果显示在植物乳杆菌Lp中,稳定值最小的是16S ribosomal RNA,用Norm Finder软件分析,稳定值最小的也是16S ribosomal RNA。揭示16S ribosomal RNA适合做植物乳杆菌正常生长状态下的内参基因。The fluorescence quantitative PCR was used to detect the gene expression level of four genes（16S r RNA,Glyceraldehyde-3-phosphate dehydrogenas,L-lactate dehydrogenase and rec A protein）in Lactobacillus plantarum Lp. The experimental results were analyzed with two kinds of software ge Norm and Norm Finder program. The result showed that the stable minimum value was 16 s rRNA in the two software. 16 s rRNA was suitable for the reference gene in Lactobacillus plantarum Lp.

关 键 词：实时荧光定量PCR 植物乳杆菌Lp 内参基因 

分 类 号：Q78[生物学—分子生物学]