Foxp3 Expression in CD4＋CD25＋Foxp3＋ Regulatory T Cells Promotes Development of Colorectal Cancer by Inhibiting Tumor Immunity  

作　　者：朱晓文 朱海珍 朱尤庆 冯茂辉 齐健 陈志芬 

机构地区：[1]Department of Gastroenterology, Zhongnan Hospital of Wuhan University, Wuhan 430071, China [2]The Hubei Clinical Center & Key Laboratory of Intestinal & Colorectal Diseases, Wuhan 430071, China [3]Department of Surgical Oncology, Zhongnan Hospital of Wuhan University, Wuhan 430071, China

出　　处：《Journal of Huazhong University of Science and Technology(Medical Sciences)》2016年第5期677-682,共6页华中科技大学学报（医学英德文版）

基　　金：supported by a grant from Natural Science Foundation of Hubei Province,China(No.2009CD201)

摘　　要：The mechanism underlying CD4~＋CD25~＋Foxp3~＋ regulatory T cells（Tregs） promoting the development of colorectal cancer（CRC） was elucidated in the present study. Forty-eight cases of colorectal carcinomas, 22 cases of colon polyps and 21 cases of normal colorectal tissues were collected. The correlation among Foxp3, IL-10 and Stat3, and the clinical relevance of these three indexes were analyzed. The results showed that the levels of Foxp3 expressed in infiltrating CD4~＋CD25~＋Foxp3~＋Tregs, and IL-10 and Stat3 in CRC tissues were all significantly higher than those in polypus tissues and normal colon tissues（P〈0.01）. Pearson correlation analysis indicated that the expression level of Foxp3 was positively correlated with Stat3 at m RNA level（r=0.526, P=0.036）, and was positively correlated with IL-10 at protein level（r=0.314, P=0.030）. The Foxp3 expressed in CD4~＋CD25~＋Foxp3~＋Tregs was correlated with the histological grade, lymph node metastasis and TNM stage of CRC（P〈0.05 for all）. The IL-10 expression was correlated with the histological grade and TNM stage（both P〈0.05）. The Stat3 expression was correlated with the lymph node metastasis and TNM stage（both P〈0.05）. It was concluded that CD4~＋CD25~＋Foxp3~＋Tregs can inhibit tumor immunity in combination with some other related inhibitory cytokines and that Foxp3 expression in CD4~＋CD25~＋Foxp3~＋Tregs correlates with CRC progression.The mechanism underlying CD4~＋CD25~＋Foxp3~＋ regulatory T cells（Tregs） promoting the development of colorectal cancer（CRC） was elucidated in the present study. Forty-eight cases of colorectal carcinomas, 22 cases of colon polyps and 21 cases of normal colorectal tissues were collected. The correlation among Foxp3, IL-10 and Stat3, and the clinical relevance of these three indexes were analyzed. The results showed that the levels of Foxp3 expressed in infiltrating CD4~＋CD25~＋Foxp3~＋Tregs, and IL-10 and Stat3 in CRC tissues were all significantly higher than those in polypus tissues and normal colon tissues（P〈0.01）. Pearson correlation analysis indicated that the expression level of Foxp3 was positively correlated with Stat3 at m RNA level（r=0.526, P=0.036）, and was positively correlated with IL-10 at protein level（r=0.314, P=0.030）. The Foxp3 expressed in CD4~＋CD25~＋Foxp3~＋Tregs was correlated with the histological grade, lymph node metastasis and TNM stage of CRC（P〈0.05 for all）. The IL-10 expression was correlated with the histological grade and TNM stage（both P〈0.05）. The Stat3 expression was correlated with the lymph node metastasis and TNM stage（both P〈0.05）. It was concluded that CD4~＋CD25~＋Foxp3~＋Tregs can inhibit tumor immunity in combination with some other related inhibitory cytokines and that Foxp3 expression in CD4~＋CD25~＋Foxp3~＋Tregs correlates with CRC progression.

关 键 词：colorectal histological colon CD25＋Foxp3 metastasis infiltrating polyp carcinomas immunity inhibit 

分 类 号：R735.3[医药卫生—肿瘤]