丹参多酚酸对慢性应激大鼠抑郁相关行为改善的研究  

The effects of salvianolic acid on depression related behavior in chronic mild stress rats

作　　者：贾立娜[1] 于鲁璐[1] 李云鹏[1] 陈欠欠 甄凤亚 王硕[1] 卢文婷[1] 荣蓉[1] 赵栋[1] 许顺江 安翠霞[1] 

机构地区：[1]河北医科大学第一医院精神科河北医科大学精神卫生研究所,石家庄050031 [2]河北省脑老化与认知神经科学重点实验室

出　　处：《中华精神科杂志》2016年第5期322-327,共6页Chinese Journal of Psychiatry

基　　金：国家自然科学基金（81271489）;河北省卫生厅医学科学研究课题计划（20130277）;河北省科技厅科技支撑计划（15277713D）

摘　　要：目的 观察丹参多酚酸对慢性温和应激抑郁模型大鼠抑郁行为的影响.方法 将33只健康雄性,体质量150～200 g的清洁级Sprague Dawley （SD）大鼠按随机数字表法分为5组,分别为非应激＋生理盐水组（对照组,6只）,应激＋生理盐水组（生理盐水组,6只）,应激＋氟西汀组（氟西汀组,7只）,应激＋丹参多酚酸组（丹酚酸组,7只）,应激＋氟西汀＋丹酚酸组（联合组,7只）.除对照组外,其他4组连续21d进行慢性温和应激造模.造模成功后,每天下午各组分别腹腔注射相对应的0.9％氯化钠10 ml/kg,氟西汀20 mg/kg,丹参多酚酸40 mg/kg,丹酚酸40 mg/kg＋氟西汀20 mg/kg,体积体质量比10 ml/kg,每周进行蔗糖偏好试验,称重,给药21d后进行旷场试验、强迫游泳测试以评价药物对大鼠抑郁样行为的作用.对基线水平体质量（g）、应激21d后与给药21d后体质量、蔗糖偏好率（％）、强迫游泳后4 min静止不动时间（s）、旷场探究活动（爬格个数、站立次数、修饰次数及粪便粒数）进行单因素方差分析和LSD法两两比较.结果 （1）应激21d后,5组大鼠体质量、蔗糖偏好率、强迫游泳静止不动时间、旷场试验中爬格个数和站立次数的差异有统计学意义（F分别为3.747,14.968,8.439,38.925,7.893,均P＜0.05）,应激的4组大鼠体质量、蔗糖偏好率、爬格个数、站立次数均低于对照组,静止不动时间高于对照组,差异有统计学意义（均P＜0.05）.两两比较结果显示,应激的4组大鼠均值低于对照组（均P＜0.01）,但4组间差异无统计学意义.（2）给药21d后,5组大鼠的体质量、蔗糖偏好率、强迫游泳静止不动时间、旷场试验中站立次数和爬格数差异有统计学意义（F分别为8.179,6.609,27.608,34.149,4.341,均P＜0.01）,氟西汀组、丹酚酸组、联合组及对照组体质量、蔗糖偏好率均高于生理盐水组（均P＜0.05）,静止不动时间低于生理盐水组（P均Objective To observe the effect of salvianolic acid on the depressive behavior in rats.Methods 33 healthy male Sprague Dawley （SD） rats were divided into 5 groups,one control group with no stress challenge（n=6） and 4 chronic stress groups（saline group,n=6;fluoxetine group,n=7;salvianolic acid group,n=7;fluoxetine ＋ salvianolic acid combination group,n=7.The rats but not in control group were exposed to stress for 3 weeks,and then were daily afternoon injected intraperitoneally saline（10 ml/kg）,fluoxetine（20 mg/kg）,salvianolic acid（40 mg/kg） or combined fluoxetine and salvianolic acid,with dosage of 10 ml/kg.Sucrose preference test and the weights of rats were measured every week.Open field test （OFT）and forced swimming test （FST） were performed after chronic stress challenge and drug treatment to assess the effect of drug treatments on depressive-like symptoms.One-way ANOVA and LSD methods were adopted to compare two groups （the weight （g） of rats,the last 4 minutes duration of immobility in the forced swimming test （s）,sucrose preference values（％） and open field locomotor activities （the number of crossing grids,stand-up times,grooming times and feces） of the baseline,21days after stress exposure and 21 days after drug injection）.Results 21 days after stress exposure,differences were found in the body weights,sucrose preference values,the last 4 minutes duration of immobility in the forced swimming test and the locomotor activities for 5 minutes （the number of crossing grids and stand-up times） in the OFf （F were 3.747,14.968,8.439,38.925,7.893 respectively,all had P＜0.05）.The weights,sucrose preference values and the locomotor activities in the OFf of the 4 stress groups were lower than control group,yet the last 4minutes duration of immobility in the forced swimming test were higher than control group （all P＜0.05）.According to LSD test,the mean of the above data in the 4 stress groups were lower than control group respectively （all P＜0.0

关 键 词：抑郁症 氟西汀 疾病模型 动物 行为 丹参多酚酸 慢性温和应激模型 

分 类 号：R749.4[医药卫生—神经病学与精神病学]