N-乙酰-L-色氨酸对新生鼠脑缺血缺氧纹状体损伤的保护作用  

Protective effect of N-acetyl-L-tryptophan on striatal injury of neonatal rat hypoxic ischemic encephalopathy

作　　者：赵纳杰 王聚信[2] 李晓双[2] 高香红[2] 于海[2] 刘清华[2] 张明[2] 于树娜[2] 蒋吉英[2] 

机构地区：[1]潍坊医学院药学院,山东潍坊261053 [2]潍坊医学院人体解剖学教研室,山东潍坊261053

出　　处：《重庆医学》2016年第31期4331-4333,共3页Chongqing medicine

基　　金：山东省自然科学基金资助项目(ZR2010HM006,ZR2014HL020,ZR2014HL021);山东省教育厅资助项目(J11LF14);山东省医药卫生科技发展计划资助项目(2014WS0464);潍坊医学院科技创新研究基金资助项目(K1301001);潍坊医学院大学生科技创新基金资助项目(KX20150091);潍坊医学院青年创新基金资助项目(K1302035)

摘　　要：目的探讨N-乙酰-L-色氨酸(L-NAT)对新生鼠脑缺血缺氧纹状体损伤的保护作用。方法用出生第7天的SD大鼠制备新生鼠脑缺血缺氧模型,HE染色观察纹状体的形态学变化;免疫荧光染色观察Caspase3、Bcl-2和Bax的表达情况。结果与Sham组相比,HIE组纹状体中神经细胞排列紊乱,且数量明显减少,Caspase-3、Bcl-2、Bax的表达升高;HIE+L-NAT组纹状体细胞的损伤程度减轻,Bax、Caspase-3表达下降,Bcl-2的表达进一步升高。结论 L-NAT可能通过调节Bcl-2家族蛋白的表达减轻纹状体细胞的损伤程度,从而保护新生鼠脑缺血缺氧损伤。Objective To investigate the protective effects of N-acetyl-L-tryptophan （L-NAT） on neonatal rat striatal injury hypoxic-ischemic encephalopathy（HIE）. Methods The HIE rat model was established with SD neonatal rats on 7 d. The morphological change of striatum was evaluated by HE staining and the expression levels of Caspase3,Bcl-2 and Bax were observed by immunofluorescence staining. Results Compared with the sham group,the expression of Caspase-3,Bcl-2 and Bax in the HIE group was increased, while the neurons in striatum were arranged in disorder, moreover the number was significantly decreased;the injury degree of striatal neurons in the HIE＋ L-NAT group was alleviated,Caspase-3 and Bax expression was decreased and Bel-2 expression was further increased. Conclusion L-NAT protects neonatal rat hypoxic-ischemic cerebral injury possibly by regulating Bcl-2 family protein expression to alleviate the injury degree of striatal neurons.

关 键 词：缺血缺氧损伤 L-乙酰-N-色氨酸 纹状体 细胞凋亡 大鼠 

分 类 号：R742[医药卫生—神经病学与精神病学]