小剂量阿糖胞苷诱导神经母细胞瘤细胞分化的研究  

Study of Differentiation of Neuroblastoma SMS-KCNR cell by Low-dose Cytosine Arabinside

作　　者：罗福永 刘颖[1] 朱星华[1] 刘颖[1] 孙艳丽[1] 成晓君[1] 黄康[1] 曲梅 LUO Yong-fu LIU Ying ZHU Xin-hua et al(The Forth Affiliated Hospital of China Medical University, Shenyang 110032, China)

机构地区：[1]中国医科大学附属第四医院,辽宁沈阳110032

出　　处：《中国肿瘤》2016年第11期904-909,共6页China Cancer

基　　金：沈阳市科学技术计划项目(F13-221-9-43)

摘　　要：[目的]通过人神经母细胞瘤(NB)SMS-KCNR细胞的体外实验,观察小剂量阿糖胞苷(Ara-C)对NB细胞的诱导分化作用。[方法]MTS法检测小剂量Ara-C对SMS-KCNR细胞增殖的影响;观察小剂量Ara-C处理后SMS-KCNR细胞的形态变化;流式细胞仪检测小剂量Ara-C处理后SMS-KCNR细胞的细胞周期分布;Western blot检测Trk A、N-myc蛋白的表达,RT-PCR检测Trk A及MYCN m RNA水平的变化。[结果]小剂量Ara-C对SMS-KCNR细胞的增殖具有抑制作用,诱导SMS-KCNR细胞周期停滞于S期,G2/M期细胞比例下降(P<0.05)。经小剂量Ara-C处理后SMS-KCNR细胞形态与正常神经节细胞类似,并使Trk A的表达上调(P<0.05),MYCN的表达下调(P<0.05)。[结论]小剂量Ara-C对SMS-KCNR细胞具有诱导分化作用,该作用可能与Trk A表达上调,MYCN表达下调有关。[Purpose] Through a series of experiments of SMS-KCNR cells of neuroblastoma（NB）by low-dose cytosine arabinside（Ara-C） in vitro. To explore the differentiation of SMS-KCNR cells by low-dose Ara-C. [Methods] The proliferation of SMS-KCNR cells treated with low-dose Ara-C was detected by MTS. The morphological changes of SMS-KCNR cells was observed by microscope. The distribution of cell cycles was analyzed by Flow cytometry. Further,the protein expression of Trk A and N-myc was measured by Western blot and the m RNA change of the Trk A and MYCN was quantified by the real time-PCR. [Results] The proliferation of SMS-KCNR cells were inhibited by low-dose Ara-C. Low-dose Ara-C induced the stagnation of the cell cycle in S phase.The content of SMS-KCNR cells in G2/M phase decreased（P〈0.05）. SMS-KCNR cells’ morphology was similar to normal ganglion cells after treated by low-dose Ara-C. The expression of Trk A increased by low-dose Ara-C. but decreased the expression of MYCN. [Conclusion] The low-dose Ara-C could induce the differentiation of SMS-KCNR cells might be through increased the expression of Trk A.

关 键 词：神经母细胞瘤 SMS-KCNR细胞 小剂量阿糖胞苷 诱导分化 

分 类 号：R739.44[医药卫生—肿瘤]