药用真菌蛹虫草遗传多样性的SCoT分析  

SCoT Analysis on Genetic Diversity of Medicinal Fungus Cordyceps militaris

作　　者：李挺[1] 宋斌[1] 林敏[1] LI Ting SONG Bin LI Min(Guangdong Institute of Microbiology/State Key Laboratory of Applied Microbiology Southern China/Guangdong Provincial Key Labora- tory of Microbial Culture Collection and Application/Guangdong Open Laboratory of Applied Microbiology, Guangzhou 510070, China)

机构地区：[1]广东省微生物研究所/省部共建华南应用微生物国家重点实验室/广东省菌种保藏与应用重点实验室/广东省微生物应用新技术公共实验室,广东广州510070

出　　处：《中药材》2016年第7期1488-1492,共5页Journal of Chinese Medicinal Materials

基　　金：广州市科技计划项目(201607020017);广东省科技计划项目(2016A030303035);真菌学国家重点实验室开放基金(sklmkf201308)

摘　　要：目的：研究药用真菌蛹虫草的种质资源遗传多样性。方法：采用非加权平均距离聚类法（UPGMA）结合主坐标分析法（PCoA）对27株不同来源的蛹虫草菌株的SCoT分子标记条带进行研究。结果：从36个引物中筛选出8条重复性好、条带清晰的引物进行PCR扩增,共扩增出59条条带,其中55条具有多态性,多态性比率为94.33%,平均多态性条带数为6.875。经NTSYSpc软件分析,27株蛹虫草菌株间的遗传相似系数在0.383～0.933之间,说明SCoT标记能够揭示蛹虫草种质间较高的遗传多样性。通过UPGMA法和PCoA法分析,27株蛹虫草分别分成6组和3组,菌株的遗传多样性与其来源有一定的相关性。结论：SCoT分子标记适用于药用真菌蛹虫草种质资源的多样性研究。Objective： To analyze the genetic diversity of medicinal fungus of 27 strains of Cordyceps militaris. Methods： Unweighted pair-group method with arithmetic means（ UPGMA） and principal coordinate analysis（ PCoA） were used to determine the genetic diversity of 27 Cordyceps militaris strains based on start codon targeted polymorphism（ SCoT）. Results： Based on eight informative primers,a total of 59 bands were produced,and 94. 33% of them were polymorphic. The average of polymorphism bands was 6. 875. The results suggested that great genetic diversity existed in Cordyceps militaris. Basically,27 strains were separated into six groups using UPGMA method,while three groups when using PCoA method,which showed that genetic diversity of Cordyceps militaris samples were related to their geographical origins. Conclusion： SCoT markers are informative and could be used to detect polymorphism for medicinal fungus of Cordyceps militaris strains.

关 键 词：蛹虫草 聚类分析 主坐标分析 

分 类 号：R282.71[医药卫生—中药学]