Morphometric comparison by the ISAS CASA-DNAf system of two techniques for the evaluation of DNA fragmentation in human spermatozoa  

作　　者：Sara Sadeghi Almudena Garcia-Molina Ferran Celma Anthony Valverde Sogol Fereidounfar Caries Soler 

机构地区：[1]Department of Functional Biology and Physical Anthropology, University of Valencia, 46100 Burjassot, Valencia, Spain [2]R+D Department, Proiser R+D, Scientific Park, Universitat de Valencia, 46980 Paterna, Valencia, Spain [3]Technological Institute of Costa Rica, San Carlos Campus, School of Agronomy, 223-21001 Alajuela, Costa Rica [4]Department of Botany, Kharazmi University, Tehran, Iran

出　　处：《Asian Journal of Andrology》2016年第6期835-839,共5页亚洲男性学杂志（英文版）

摘　　要：DNA fragmentation has been shown to be one of the causes of male infertility, particularly related to repeated abortions, and different methods have been developed to analyze it. In the present study, two commercial kits based on the SCD technique （Halosperm~ and SDFA） were evaluated by the use of the DNA fragmentation module of the ISAS vl CASA system. Seven semen samples from volunteers were analyzed. To compare the results between techniques, the Kruskal-Wallis test was used. Data were used for calculation of Principal Components （two PCs were obtained）, and subsequent subpopulations were identified using the Halo, Halo/Core Ratio, and PC data. Results from both kits were significantly different （P 〈 0.001）. In each case, four subpopulations were obtained, independently of the classification method used. The distribution of subpopulations differed depending on the kit used. From the PC data, a discriminant analysis matrix was obtained and a good a posterioriclassification was obtained （97.1% for Halosperm and 96.6% for SDFA）. The present results are the first approach on morphometric evaluation of DNA fragmentation from the SCD technique. This approach could be used for the future definition of a classification matrix surpassing the current subjective evaluation of this important sperm factor.DNA fragmentation has been shown to be one of the causes of male infertility, particularly related to repeated abortions, and different methods have been developed to analyze it. In the present study, two commercial kits based on the SCD technique （Halosperm~ and SDFA） were evaluated by the use of the DNA fragmentation module of the ISAS vl CASA system. Seven semen samples from volunteers were analyzed. To compare the results between techniques, the Kruskal-Wallis test was used. Data were used for calculation of Principal Components （two PCs were obtained）, and subsequent subpopulations were identified using the Halo, Halo/Core Ratio, and PC data. Results from both kits were significantly different （P 〈 0.001）. In each case, four subpopulations were obtained, independently of the classification method used. The distribution of subpopulations differed depending on the kit used. From the PC data, a discriminant analysis matrix was obtained and a good a posterioriclassification was obtained （97.1% for Halosperm and 96.6% for SDFA）. The present results are the first approach on morphometric evaluation of DNA fragmentation from the SCD technique. This approach could be used for the future definition of a classification matrix surpassing the current subjective evaluation of this important sperm factor.

关 键 词：DNA fragmentation Halosperm MORPHOMETRY principal component analysis SDFA SUBPOPULATION 

分 类 号：Q523[生物学—生物化学] TP391.7[自动化与计算机技术—计算机应用技术]