肺鳞状细胞癌血清CCL11表达及临床意义研究  

The serum level and its prognostic significance of CCL11 in human lung squamous cell carcinoma

作　　者：陈天宇[1] 秦少云 姜克家[1] 戴莹[1] 

机构地区：[1]江苏泰州市人民医院南院呼吸科,江苏泰州225300

出　　处：《临床和实验医学杂志》2016年第21期2107-2110,共4页Journal of Clinical and Experimental Medicine

摘　　要：目的探讨CC修饰趋化因子11(CCL11)在肺鳞状细胞癌(LSCC)患者血清中的表达情况及临床意义。方法分别采用逆转录定量PCR(qRT-PCR)和酶联免疫吸附(ELISA)法检测90例术前肺鳞状细胞癌及30例健康志愿者血清样本中CCL11 mRNA及蛋白的表达,分析血清CCL11蛋白表达与患者临床特征及预后间的相关性。结果CCL11在肺鳞状细胞癌患者血清中高表达(P<0.05),并与肿瘤淋巴结转移(t=1.871,P=0.022)及高TNM分期(III+IV期,t=2.219,P=0.015)呈显著正相关;血清CCL11蛋白高表达的患者3年生存率低于CCL11蛋白表达阴性患者(P<0.05)。结论 CCL11在肺鳞状细胞癌患者血清中表达上调,血清高水平CCL11蛋白表达与肿瘤恶性临床病理特征及不良预后有关。Objective To investigate the serum level of C - C motif chemokine 11 （ CCL11 ） and its prognostic significance in human lung squamous cell carcinoma （LSCC）. Methods 90 LSCC patients and 30 normal volunteers serum samples were collected between March 2011 and March 2012. The mRNA and protein expression of CCL11 were detected by qRT- PCR and ELISA. The relationship between CCL11 and clinical features were analyzed. Results The serum expression level of CCLll was significantly higher in LSCC patients than that in normal volunteers （ P 〈 0.05 ）. The high expression of CCL11 protein was positively correlated with lymphatic metastasis and advanced TNM stage （III ＋ IV stage, P 〈0.05）. Both the 3 -year overall survival rate and disease -free survival rate were lower in CCLll positive expression group than those in CCL11 negative expression group. Conclusion The serum level of CCL11 is significantly higher in lung squamous cell carcinoma tissues than that in tumor adjacent tissues, and the high level of CCL11 is associated with malignant clinicopathological features and lower survival rate. CCL11 may be an effective diagnosis and prognosis marker for LSCC patients.

关 键 词：肺鳞状细胞癌 CCL11 表达 预后 

分 类 号：R734.2[医药卫生—肿瘤]