NEP1-40对局灶性脑缺血大鼠生长相关蛋白43和微管相关蛋白2表达的影响  

Effect of NEP1-40 on expression of GAP-43 and MAP-2 in rats following focal cerebral ischemia

作　　者：赵红[1] 刘振东[2] 王海丰 蔺建文[1] 赵红玲[1] 王苏平[1] 

机构地区：[1]大连市中心医院神经内一科,116033 [2]大连医科大学研究生 [3]大连市旅顺口区人民医院麻醉科

出　　处：《中华老年心脑血管病杂志》2016年第11期1204-1207,共4页Chinese Journal of Geriatric Heart,Brain and Vessel Diseases

摘　　要：目的观察局灶性脑缺血大鼠生长相关蛋白43(GAP-43)和微管相关蛋白2(MAP-2)的表达变化,及轴突生长抑制因子A(NogoA)拮抗剂NEP1-40给药后对其表达的影响,探讨NogoA抑制神经再生的可能机制。方法雄性SD大鼠48只,随机分为3组:假手术组、模型组和给药组,每组16只,采用线栓法建立大脑中动脉闭塞模型,给药组于脑缺血术后1d给予NEP1-40溶液侧脑室注射。术后1、3、7和14d用免疫组织化学法检测缺血侧纹状体区域GAP-43和MAP-2的表达变化。结果脑缺血后缺血侧纹状体区域,在模型组和给药组脑缺血后1、3和7d GAP-43阳性表达的吸光度值逐步增高,14dGAP-43表达有所下降,但均明显高于假手术组。给药组1、3、7和14dGAP-43阳性表达的吸光度值均明显高于模型组(0.19±0.18 vs 0.17±0.23,0.24±0.10 vs 0.21±0.04,0.30±0.21 vs 0.25±0.06和0.26±0.23 vs 0.23±0.08,P<0.05)。模型组和给药组各时间点MAP-2阳性表达的吸光度值逐渐升高,但均明显低于假手术组。给药组各时间点MAP-2阳性表达的吸光度值均明显高于模型组。结论抑制NogoA后可以通过增加缺血区GAP-43和MAP-2的表达,促进神经修复,这可能是NogoA抑制神经再生的机制。Objective To study the effect of NEP1-40 on expression of GAP-43 and MAP-2in rats following focal cerebral ischemia.Methods Forty-eight male SD rats were divided into sham operation group,MCAO model group and NEP1-40 treatment group（16in each group）.A MCAO model was established by Longa occulsion.The rats in NEP1-40 treatment group received intracerebroventricular injection of NEP1-40 on day 1after operation.Expression of GAP-43 and MAP-2in ischemic striatum was detected with immunohistochemical staining on days 1,3,7and 14 respectively after operation.Results The expression level of GAP-43 gradualy increased in MCAO group and NEP1-40 treatment group on days 1,3and 7after operation and then decreased on day14 after operation and was always significantly higher than in sham group.The expression level of GAP-43 was significantly higher in NEP1-40 treatment group than in MCAO model group on days1,3,7and 14 after operation.The expression level of MAP-2was significantly higher in MCAO model group and NEP1-40 treatment group than in sham operation group and in NEP1-40 treatment group than in MCAO model group at different time points after operation（P〈0.05）.Conclusion NogoA can upregulate the expression level of GAP-43 and MAP-2and can thus promote nerve repair possibly by inhibiting the nerve regeneration.

关 键 词：脑缺血 GAP-43蛋白 微管相关蛋白质类 神经生长因子类 

分 类 号：R743.3[医药卫生—神经病学与精神病学]