可乐猪乳头数性状全基因组关联分析  

Genome-wide Association Study of Teats Number Traits in Kele Pigs

作　　者：谢健[1] 喻昌燕 许瑶[1] 牛熙[2] 冉雪琴[3] 王嘉福[2,4] XIE Jian YU Chang-yan XU Yao NIU Xi RAN Xue-qin WANG Jia-fu(College of Life Sciences, Guizhou University, Guiyang 550025, China Institute of Agro-bioengineering , Guizhou University, Guiyang 550025, China College of Animal Science, Guizhou University, Guiyang 550025, China Tongren University, Tongren 554300, China)

机构地区：[1]贵州大学生命科学学院,贵阳550025 [2]贵州大学农业生物工程研究院,贵阳550025 [3]贵州大学动物科学学院,贵阳550025 [4]铜仁学院,铜仁554300

出　　处：《中国畜牧兽医》2016年第11期2866-2872,共7页China Animal Husbandry & Veterinary Medicine

基　　金：国家高技术研究发展计划(863计划)课题(2013AA102503);贵州大学创新基金(2015009);贵州大学"本科教学工程"项目(BKJX2012007);贵州省农业攻关项目(黔科合NY[2013]3073号);贵州省科技创新人才团队建设专项(黔科合人才团队2009-4006)

摘　　要：乳头数性状是猪最重要的繁殖性状之一,与养猪业的经济效益密切相关。本试验以Illunima Porcine SNP60K芯片对22头可乐猪进行基因分型,通过Plink 1.07软件,基于混合线性模型对左乳头数、右乳头数和总乳头数性状进行全基因组关联分析。经过严格的质量控制和多重检验之后,共鉴定出全基因组水平潜在关联的SNPs位点4个(P<2.06E-5);染色体水平显著关联位点3个,潜在关联位点18个;在关联SNP位点可能连锁的上、下游1cM区间内检索到304个基因,其中Wnt及Fgf信号通路中的候选基因BTRC、FGF5、FGF8和BMP3、RASGEF1B、HMGB3可能影响猪的乳头数性状或繁殖性状。通过全基因组关联分析策略发掘出的关联SNP位点及潜在候选基因,将为可乐猪的选育保种奠定基础。Teats number is one of the most important reproductive traits,and closely related to the economic benefit in pig industry.In order to reveal the underlying genetics of left teats number,right teats number and total teats number traits,agenome-wide association study（GWAS）was performed.Samples of DNA were collected to genotyping for 22 Kele pigs using the Illumina Porcine SNP 60 KChip.The GWAS was performed using a mixed-effects model and linear regression approach.When a genome-wide threshold was determined using the Bonferroni method（P 〈2.06E-5）,4single nucleotide polymorphism（SNP）markers were potentially associated with left teats number,right teats number and total teat number.However,3SNPs were significant associated and 18 SNPs were potentially associated in chromosomes level.304 Ensembl genes were retrieved around 1cM of the associated SNPs.The candidate genes in Wnt and Fgf signaling pathway（BTRC,FGF5,FGF8,BMP3,RASGEF1 Band HMGB3）might have effect on target traits.These results provided valuable information about the selective breeding for Kele pigs.

关 键 词：SNP芯片 全基因组关联分析 乳头数 

分 类 号：Q78[生物学—分子生物学]