钙对赭曲霉毒素A(OTA)诱导拟南芥毒性的抑制作用  

Calcium Inhibits Ochratoxin A(OTA) Induced Arabidopsis thaliana Toxicity

作　　者：吕杨俊 郝俊冉[1] 许文涛[1,3] 张海华[2] 翟亚楠[1] 杨静[1] LV Yang-Ju HAO Jun-Ran XU Wen-Tao ZHANG Hai-Hua ZHAI Ya-Nan YANG Jing(College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, China Hangzhou Tea Research Institute, All China Federation of Supply and Marketing Cooperatives, Hangzhou 310016, China The Supervision, Inspection ＆Testing Center of Genetically Modified Orginisms Food Safety, Ministry of Agriculture, Beijing 100083, China)

机构地区：[1]中国农业大学食品科学与营养工程学院,北京100083 [2]中华全国供销合作总社杭州茶叶研究所,杭州310016 [3]农业部转基因生物食用安全检验监督测试中心,北京100083

出　　处：《农业生物技术学报》2016年第12期1839-1845,共7页Journal of Agricultural Biotechnology

基　　金：北京市科技计划项目(No.D121100003112001和No.D121100003112004)

摘　　要：为丰富赭曲霉毒素A(ochratoxin A,OTA)的植物毒性及其作用机理,探究钙在OTA诱导的植物毒性调控作用机理,本研究采用添加外源Ca^(2+),钙离子螯合剂乙二醇双(2-氨基乙基醚)四乙酸(ethylenebis(oxyethylenenitrilo)tetraacetic acid,EGTA)及OTA等处理离体拟南芥(Arabidopsis thaliana)叶片,通过形态学观察,叶片相对电导率、活性氧含量(reactive oxygen species,ROS)和丙二醛(malondialdehyde,MDA)含量的测定等研究钙对OTA诱导的拟南芥毒性的影响,结果表明,在一定浓度范围内(0~50 mmol/L),外源Ca^(2+)单独处理,拟南芥叶片形态学无明显变化,EGTA与OTA分别处理均会引起拟南芥叶片失绿、坏死病斑的形成,而Ca^(2+)与OTA共同处理,能显著抑制OTA诱导的拟南芥叶片失绿、坏死病斑的形成,EGTA加剧OTA的毒性作用;OTA处理使拟南芥叶片相对电导率升高,细胞膜通透性增大,外源钙能显著抑制OTA引起的拟南芥叶片相对电导率升高(P<0.05),20 mmol/L Ca^(2+)的抑制作用最强,抑制率为61.32%。此外,OTA处理使拟南芥叶片细胞ROS的爆发及MDA的积累,对拟南芥造成氧化损伤,钙能显著抑制OTA诱导的拟南芥ROS爆发(P<0.05),减少MDA的生成,抑制率分别为29.7%和71.4%。研究结果说明OTA能诱导拟南芥的植物毒性,钙能显著抑制OTA诱导的拟南芥植物毒性,钙在拟南芥受外界胁迫中起重要作用。In order to enrich the toxicity and its mechanism of ochratoxin A（OTA） in plants and explore the role of calcium in OTA toxicity to plants, this research studied the effect of calcium on the toxicity induced by OTA in Arabidopsis thaliana, by the observation of morphological changes, relative leakage rate of leaves, the content of reactive oxygen species（ROS） and malondialdehyde（MDA） under treatment with exogenous Ca-（2＋）,the calcium chelator ethylenebis（oxyethylenenitrilo） tetraacetic acid（EGTA） and OTA in vitro leaves of A.thaliana. The results showed that in a certain range of concentration, under the treatment of exogenous calcium, there was no significant change in leaf morphology of A. thaliana. EGTA and OTA both led to the formation of A. thaliana leaves chlorosis and necrosis of the lesion. Ca-（2＋） and OTA treatment together could significantly inhibit the formation of A. thaliana leaves chlorosis and necrotic lesion produced induced by OTA. EGTA treatment could aggravate those effects induced by OTA. OTA treatment increased relative leakage rate and permeability of cell membrane of A. thaliana leaves. Exogenous calcium could significantly inhibit OTA induced increase in relative leakage rate of A. thaliana leaves（P〈0.05）, 20 mmol/L Ca2＋ has the strongest inhibitory effect of which the inhibition rate was 61.32%. In addition, OTA treatment brought the outbreak of ROS and the accumulation of MDA in A. thaliana leaves, and therefore caused oxidative damage.Ca-（2＋）could effectively relieve the OTA induced A. thaliana ROS burst and reduce MDA generation（P〈0.05）;the inhibition rates were 29.7% and 71.4%, respectively. It could be concluded that OTA can induce plant toxicity in A. thaliana, calcium significantly inhibts the toxicity of OTA in A. thaliana, and plays an important role in A. thaliana＇s stress resistance.

关 键 词：赭曲霉毒素A(OTA) CA2+ EGTA 拟南芥 毒性 

分 类 号：S312[农业科学—作物栽培与耕作技术]