Wnt5a基因在乳腺癌细胞株MCF-7中的表达及其与E-钙黏蛋白、波形蛋白基因的关系  

Expression of Wnt5a gene in breast cancer cell line MCF-7 and its relationship with E-cadherin or vimentin

作　　者：邱霞 杨国荣[2] 刘利[2] 黄勇[2] 曾若兰[2] 姚晓艺[2] 石静[2] 吴至佛 汪灵[2] 刘桂[3] 蔡鑫嘉 黄俊辉[2] Qiu Xia Yang Guorong Liu Li Huang Yong Zeng Ruolan Yao Xiaoyi Shi Jing Wu Zhifo Wang Ling Liu Gui Cai Xinjia Huang Junhui(Department of Geriatrics, Chartgsha NO.2 Social Welfare House, Changsha 410007, China Department of Oncology, Xiangya Hospital of Central South University, Changsha 410008, China Department of Pathology, Xiangya Stomatological Hospital of Central South University, Changsha 410000, China)

机构地区：[1]长沙市第二社会福利院老年科,410007 [2]中南大学湘雅医院肿瘤科,长沙410008 [3]中南大学湘雅口腔医院病理科,长沙410000

出　　处：《肿瘤研究与临床》2016年第10期649-653,共5页Cancer Research and Clinic

基　　金：湖南省自然科学基金（11jj3122）;湖南省中医药管理局项目（2013115）;长沙市科技计划（K1406035-11）

摘　　要：目的：探讨Wnt5a基因在人乳腺癌细胞株MCF-7中的表达及其与E-钙黏蛋白、波形蛋白基因表达的相关性。方法采用反转录聚合酶链反应（RT-PCR）检测Wnt5a、E-钙黏蛋白mRNA和波形蛋白mRNA在MCF-7细胞和人正常乳腺上皮细胞株MCF-10A中的表达，分析Wnt5a表达与E-钙黏蛋白mRNA、波形蛋白mRNA的关系。结果 MCF-7细胞中，Wnt5a、E-钙黏蛋白mRNA水平均低于MCF-10A细胞[（16.93±2.97）%比（27.47±2.76）%，（12.97±1.35）%比（20.43±2.60）%，均P＜0.05]，波形蛋白mRNA水平高于MCF-10A细胞[（16.53±0.85）%比（6.33±2.08）%，P＜0.05]。在MCF-7细胞中，Wnt5a与E-钙黏蛋白mRNA的表达呈正相关（r＝0.997，P＜0.05），与波形蛋白mRNA的表达呈负相关（r＝-0.998， P＜0.05）。结论 Wnt5a在人乳腺癌细胞株MCF-7中的表达低于人正常乳腺上皮细胞株MCF-10A，其在乳腺癌的发生与发展中可能发挥抑癌基因的作用。 Wnt5a在乳腺癌细胞中的表达与E-钙黏蛋白mRNA的表达呈正相关，与波形蛋白mRNA的表达呈负相关。 Wnt5a可能通过影响乳腺癌上皮细胞的转化导致乳腺癌的侵袭和转移。Objective To investigate the relationship between Wnt5a gene and E-cadherin or vimentin gene in breast cancer cell line MCF-7. Methods RT-PCR was used to detect the mRNA expression of Wnt5a, E-cadherin and vimentin in breast cancer MCF-7 cells and the normal human mammary epithelial cell line MCF-10A, respectively, and their correlation was analyzed. Results The mRNA expression levels of Wnt5a and E-cadherin in cell line MCF-7 were significantly lower than those in cell line MCF-10A [（16.93± 2.97）%vs. （27.47±2.76） %, （12.97±1.35） % vs. （20.43±2.60） %, both P〈0.05]. The mRNA expression level of vimentin in cell line MCF-7 was significantly higher than that in cell line MCF-10A [（16.53±0.85）%（6.33± 2.08） %, P〈0.05 ]. In cell line MCF-7, the expression of Wnt5a was positively related to E-cadherin （г=0.997, P〈0.05）, but it was negatively related to vimentin （г=-0.998, P〈0.05）. Conclusions The expression of Wnt5a in human breast cancer cell line MCF-7 is significantly lower than that in cell line MCF-10A, which indicates that Wnt5a is a cancer suppressor gene in breast cancer. The expression of Wnt5a in cell line MCF-7 is positively related with E-cadherin, and it is negatively related with vimentin. Wnt5a may cause invasion and metastasis of breast cancer cell through the breast epithelial mesenchymal transitions.

关 键 词：乳腺肿瘤 E-钙黏蛋白 波形蛋白 上皮间质转化 

分 类 号：R737.9[医药卫生—肿瘤]