基于微流控技术的微生物细胞梯度稀释分离方法  

A Microfluidic Gradient-Dilution Chip for Rapid Microbe Cell Separation

作　　者：徐娟[1] 蓝英[1] 潘迎捷[1,2,3] 赵勇[1,2,3] 张炜佳[1,2,3] 

机构地区：[1]上海海洋大学食品学院,上海201306 [2]上海水产品加工及贮藏工程技术研究中心,上海201306 [3]农业部水产品贮藏保鲜质量安全风险评估实验室(上海),上海201306

出　　处：《微生物学杂志》2016年第5期97-102,共6页Journal of Microbiology

基　　金：国家自然科学基金项目(31501555);上海市科委项目(14DZ1205100;14320502100);浦江人才计划项目(14PJ1404300);上海市科技兴农重点攻关项目(沪农科攻字2015第4-8号)

摘　　要：随着微流控分析技术的快速发展,集成化的微流控芯片在满足实验高通量的同时,还在微生物细胞分离领域呈现出独特的优势。本研究基于微流控技术,制备了以聚二甲基硅氧烷(PDMS)、玻片为材料的细菌细胞梯度稀释分离芯片。该芯片的核心是通过一系列复杂的梯度网络来实现对细菌悬液的连续稀释,最终被分离的细菌细胞进入通道末端的存储孔内。结果显示,该方法能分离出的最少细菌细胞数低于10个。此芯片平台操作简单、耗时短、成本低,为微生物单细胞研究提供了新的途径。Along with the rapid advances in microfluidic technology,the integrated microfluidic chip has the potential to meet the demand of high-throughput experimentations in lab and especially presents the special superiority in the field of microbe cells separation. Based on the microfluidic technology, a polydimethoxysilane（ PDMS）-glass microchip was prepared in this study which contains a network of gradient-forming channels for bacteria cells separation. The core of the microchip is to realize a successive dilution process by means of a series of complicated gradient network and finally the isolated bacteria cells entered to the end of the channel. The results showed that the number of separated bacteria cells by this method could be separated at minimum of less than ten. This chip platform is simple to operate,short time consuming,and low cost,which provide a new pathway to study microbe single-cell.

关 键 词：微流控芯片 微生物细胞分离 连续梯度稀释 

分 类 号：Q-31[生物学]