注射液中酚磺乙胺及焦亚硫酸钠含量的同时测定  

Simultaneous determination of etamsylate and sodium pyrosulfite in injection

作　　者：杨凤珍[1] 袁华[1] 范小振[1] 张文育[1] YANG Feng- zhen YUAN Hua FAN Xiao- zhen ZH ANG Wen-yu(College of Chemistry and Chemical engineering,Cangzhou Normal University,Cangzhou 061001,China)

机构地区：[1]沧州师范学院化学与化工学院,河北沧州061001

出　　处：《化学研究与应用》2016年第11期1610-1613,共4页Chemical Research and Application

基　　金：河北省科技支撑项目(13274803)资助

摘　　要：酚磺乙胺及焦亚硫酸钠均在酸性的吐温80-罗丹明6G体系中产生化学发光,但具体发光条件不同,因此利用化学发光分析法,在同一体系中根据不同条件可对针剂注射液中酚磺乙胺及焦亚硫酸钠的含量进行同时测定。酚磺乙胺的质量浓度在0.05~3.0μg·mL^(-1)范围内与光信号呈现良好的线性关系,检出限为0.01μg·mL^(-1),相对标准偏差小于3.5%(n=5)。测定焦亚硫酸钠的工作曲线线性范围为0.07~5.0μg·mL^(-1),检出限为0.02μg·mL^(-1),相对标准偏差小于4.2%(n=5)。该测定方法可用于针剂药物中酚磺乙胺及焦亚硫酸钠的含量测定,结果令人满意。The chemiluminescence emission was generated by mixing etamsylate or sodium pyrosulfite with acidic tween80-rhodamine 6G.The specific luminescent conditions were different,so the content of etamsylate and sodium pyrosulfite could be determined in the same chemiluminescence system according to the different conditions.The quantitation range was from 0. 05 to 3. 0μg·mL^-1with a detection limit of 0. 01 μg·mL^-1,and a relative standard deviation of less than 3. 5%（ n = 5） for etamsylate.The working curve liner range was 0. 07 to 5. 0μg·mL^-1with a detection limit of 0. 02 μg·mL^-1,and arelative standard deviation of less than4. 2%（ n = 5） for the determination of sodium pyrosulfite.The proposed method was applied to the quantitation of etamsylate and sodium pyrosulfite in injection with satisfactory results.

关 键 词：化学发光 酚磺乙胺 焦亚硫酸钠 吐温80 罗丹明6G 

分 类 号：O657.3[理学—分析化学]